RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR.

Autor: Yang W; Department of Infectious Diseases, Infectious Diseases and Pathogen Biology Center, The First Hospital of Jilin University, Changchun, China.; Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China.; Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, China., Wang H; Department of Infectious Diseases, Infectious Diseases and Pathogen Biology Center, The First Hospital of Jilin University, Changchun, China.; Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China.; Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, China., Li Z; Department of Infectious Diseases, Infectious Diseases and Pathogen Biology Center, The First Hospital of Jilin University, Changchun, China.; Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China.; Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, China., Zhang L; State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China., Liu J; State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, China., Kirchhoff F; Institute of Molecular Virology, Ulm University Medical Center, 89081, Ulm, Germany., Huan C; Department of Infectious Diseases, Infectious Diseases and Pathogen Biology Center, The First Hospital of Jilin University, Changchun, China. lmsxsml@jlu.edu.cn.; Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China. lmsxsml@jlu.edu.cn.; Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, China. lmsxsml@jlu.edu.cn., Zhang W; Department of Infectious Diseases, Infectious Diseases and Pathogen Biology Center, The First Hospital of Jilin University, Changchun, China. zhangwenyan@jlu.edu.cn.; Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, China. zhangwenyan@jlu.edu.cn.; Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, China. zhangwenyan@jlu.edu.cn.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Jun 21; Vol. 15 (1), pp. 5290. Date of Electronic Publication: 2024 Jun 21.
DOI: 10.1038/s41467-024-49622-1
Abstrakt: Long-term non-progressors (LTNPs) of HIV-1 infection may provide important insights into mechanisms involved in viral control and pathogenesis. Here, our results suggest that the ribosomal protein lateral stalk subunit P1 (RPLP1) is expressed at higher levels in LTNPs compared to regular progressors (RPs). Functionally, RPLP1 inhibits transcription of clade B HIV-1 strains by occupying the C/EBPβ binding sites in the viral long terminal repeat (LTR). This interaction requires the α-helixes 2 and 4 domains of RPLP1 and is evaded by HIV-1 group M subtype C and group N, O and P strains that do not require C/EBPβ for transcription. We further demonstrate that HIV-1-induced translocation of RPLP1 from the cytoplasm to the nucleus is essential for antiviral activity. Finally, knock-down of RPLP1 promotes reactivation of latent HIV-1 proviruses. Thus, RPLP1 may play a role in the maintenance of HIV-1 latency and resistance to RPLP1 restriction may contribute to the effective spread of clade C HIV-1 strains.
(© 2024. The Author(s).)
Databáze: MEDLINE
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