Phytoconstituent-derived zingerone nanoparticles disrupt the cell adhesion mechanism and suppress cell motility in melanoma B16F10 cells.

Autor: Chu LW; Department of Nursing, and Department of Cosmetic Application and Management, Yuh-Ing Junior College of Health Care and Management, Kaohsiung, Taiwan., Chen JY; Division of Neurosurgery, Fooyin University Hospital, Pingtung, Taiwan; Department of Nursing, Fooyin University, Kaohsiung, Taiwan., Chen YW; Departments of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan., Hsieh S; Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan., Kung ML; Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. Electronic address: ml@vghks.gov.tw.
Jazyk: angličtina
Zdroj: Journal of biotechnology [J Biotechnol] 2024 Sep 10; Vol. 392, pp. 48-58. Date of Electronic Publication: 2024 Jun 19.
DOI: 10.1016/j.jbiotec.2024.06.015
Abstrakt: Combining phytochemicals and nanotechnology to improve the unfavorable innate properties of phytochemicals and develop them into potent nanomedicines to enhance antitumor efficacy has become a novel strategy for cancer chemoprevention. Melanoma is the most aggressive, metastatic, and deadly disease of the primary cutaneous neoplasms. In this study, we fabricated phytoconstituent-derived zingerone nanoparticles (NPs) and validated their effects on cell adhesion and motility in melanoma B16F10 cells. Our data indicated that zingerone NPs significantly induced cytotoxicity and anti-colony formation and inhibited cell migration and invasion. Moreover, zingerone NPs dramatically interfered with the cytoskeletal reorganization and markedly delayed the period of cell adhesion. Our results also revealed that zingerone NPs-mediated downregulation of MMPs (matrix metalloproteinases) activity is associated with inhibiting cell adhesion and motility. We further evaluated the effects of zingerone NPs on Src/FAK /Paxillin signaling, our data showed that zingerone NPs significantly inhibited the protein activities of Src, FAK, and Paxillin, indicating that they play important roles in zingerone NP-mediated anti-motility and anti-invasion in melanoma cells. Accordingly, the phytoconstituent-zingerone NPs can strengthen the inhibition of tumor growth, invasion, and metastasis in malignant melanoma. Altogether, these multi-pharmacological benefits of zingerone NPs will effectively achieve the purpose of melanoma prevention and invasion inhibition.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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