Marine eDNA sampling from submerged surfaces with paint rollers.

Autor: Jarman S; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; eDNA Frontiers, Curtin University, Bentley, WA, Australia. Electronic address: Simon.Jarman@curtin.edu.au., Alexander JB; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia., Dawkins KL; eDNA Frontiers, Curtin University, Bentley, WA, Australia., Lukehurst SS; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia., Nester GM; Minderoo-UWA Deep Sea Research Centre, University of Western Australia, Crawley, WA, Australia., Wilkinson S; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Wilderlab, Miramar, Wellington, New Zealand., Marnane MJ; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Chevron Technical Center, Perth, Western Australia, Australia., McDonald JI; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Sustainability and Biosecurity, Department of Primary Industries and Regional Development (DPIRD), Hillarys, 6025, Western Australia, Australia., Elsdon TS; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Chevron Technical Center, Perth, Western Australia, Australia., Harvey ES; School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
Jazyk: angličtina
Zdroj: Marine genomics [Mar Genomics] 2024 Aug; Vol. 76, pp. 101127. Date of Electronic Publication: 2024 Jun 20.
DOI: 10.1016/j.margen.2024.101127
Abstrakt: Environmental DNA (eDNA) analyses of species present in marine environments is the most effective biological diversity measurement tool currently available. eDNA sampling methods are an intrinsically important part of the eDNA biodiversity analysis process. Identification and development of eDNA sampling methods that are as rapid, affordable, versatile and practical as possible will improve rates of detection of marine species. Optimal outcomes of eDNA biodiversity surveys come from studies employing high levels of sampling replication, so any methods that make sampling faster and cheaper will improve scientific outcomes. eDNA sampling methods that can be applied more widely will also enable sampling from a greater range of marine surface micro-habitats, resulting in detection of a wider range of organisms. In this study, we compared diversity detection by several methods for sampling eDNA from submerged marine surfaces: polyurethane foam, nylon swabs, microfibre paint rollers, and sediment scoops. All of the methods produced a diverse range of species identifications, with >250 multicellular species represented by eDNA at the study site. We found that widely-available small paint rollers were an effective, readily available and affordable method for sampling eDNA from underwater marine surfaces. This approach enables the sampling of marine eDNA using extended poles, or potentially by remotely operated vehicles, where surface sampling by hand is impractical.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Michael Marnane reports a relationship with Chevron Energy Technology Co that includes: employment. Euan S Harvey reports a relationship with Chevron Energy Technology Co that includes: funding grants. Jason B Alexander reports financial support, article publishing charges, equipment, drugs, or supplies, and writing assistance were provided by Chevron Energy Technology Co. Travis Elsdon reports a relationship with Chevron Energy Technology Co that includes: employment. Sherralee S Lukehurst reports a relationship with BHP Group Ltd. that includes: funding grants. Simon Jarman reports a relationship with Chevron Energy Technology Co that includes: funding grants.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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