Bioconversion of lignans in flaxseed cake by fermented tofu microbiota and isolation of Enterococcus faecium strain ZB26 responsible for converting secoisolariciresinol diglucoside to enterodiol.

Autor: Zhu J; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: jiaqi1109@stu2020.jnu.edu.cn., Cai Z; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: caizz@jnu.edu.cn., Song Z; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Department of Plant Sciences, University of Saskatchewan, 51 Campus Dr., Saskatoon, SK, S7N 5A8, Canada. Electronic address: ziliang.song@usask.ca., Li Y; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China., Shim YY; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Department of Plant Sciences, University of Saskatchewan, 51 Campus Dr., Saskatoon, SK, S7N 5A8, Canada. Electronic address: younyoung.shim@usask.ca., Reaney MJT; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Department of Plant Sciences, University of Saskatchewan, 51 Campus Dr., Saskatoon, SK, S7N 5A8, Canada. Electronic address: mjr997@mail.usask.ca., Lee YY; School of Science, Monash University, Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor. Electronic address: lee.yeeying@monash.edu., Wang Y; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: twyong@jnu.edu.cn., Zhang N; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition, and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: tzhning@jnu.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Nov 01; Vol. 457, pp. 140077. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1016/j.foodchem.2024.140077
Abstrakt: Human intestinal microbiota plays a crucial role in converting secoisolariciresinol diglucoside, a lignan found in flaxseed, to enterodiol, which has a range of health benefits: antioxidative, antitumor, and estrogenic/anti-estrogenic effects. Given the high secoisolariciresinol diglucoside content in flaxseed cake, this study investigated the potential of co-fermenting flaxseed cake with fermented soybean product to isolate bacterial strains that effectively convert secoisolariciresinol diglucoside to enterodiol in a controlled environment (in vitro). The co-fermentation process with stinky tofu microbiota significantly altered the lignan, generating 12 intermediate lignan metabolites as identified by targeted metabolomics. One particular promising strain, ZB26, demonstrated an impressive ability to convert secoisolariciresinol diglucoside. It achieved a conversion rate of 87.42 ± 0.33%, with secoisolariciresinol and enterodiol generation rates of 94.22 ± 0.51% and 2.91 ± 0.03%, respectively. Further optimization revealed, under specific conditions (0.5 mM secoisolariciresinol diglucoside, pH 8, 30 °C for 3 days), ZB26 could convert an even higher percentage (97.75 ± 0.05%) of the secoisolariciresinol diglucoside to generate secoisolariciresinol (103.02 ± 0.16%) and enterodiol (3.18 ± 0.31%). These findings suggest that the identified strains ZB26 have promising potential for developing functional foods and ingredients enriched with lignans.
Competing Interests: Declaration of competing interest We have no conflicts of interest concerning the work described in this manuscript.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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