Integration mapping of cardiac fibroblast single-cell transcriptomes elucidates cellular principles of fibrosis in diverse pathologies.

Autor:	Patrick R; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.; School of Clinical Medicine, UNSW Sydney, Kensington, NSW 2052, Australia.; Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia., Janbandhu V; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.; School of Clinical Medicine, UNSW Sydney, Kensington, NSW 2052, Australia., Tallapragada V; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia., Tan SSM; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia., McKinna EE; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.; Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW 2145, Australia., Contreras O; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.; School of Clinical Medicine, UNSW Sydney, Kensington, NSW 2052, Australia., Ghazanfar S; School of Mathematics and Statistics, The University of Sydney, Camperdown, NSW 2006, Australia.; Charles Perkins Centre, The University of Sydney, Camperdown, NSW 2006, Australia.; Sydney Precision Data Science Centre, The University of Sydney, Camperdown, NSW 2006, Australia., Humphreys DT; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.; School of Clinical Medicine, UNSW Sydney, Kensington, NSW 2052, Australia., Murray NJ; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.; School of Clinical Medicine, UNSW Sydney, Kensington, NSW 2052, Australia., Tran YTH; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia., Hume RD; Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW 2145, Australia.; School of Medical Science, The University of Sydney, Camperdown, NSW 2006, Australia.; Centre for Heart Failure and Diseases of the Aorta, The Baird Institute, Sydney, NSW 2042, Australia., Chong JJH; Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW 2145, Australia.; Department of Cardiology, Westmead Hospital, Westmead, NSW 2145, Australia., Harvey RP; Victor Chang Cardiac Research Institute, Darlinghurst, NSW 2010, Australia.; School of Clinical Medicine, UNSW Sydney, Kensington, NSW 2052, Australia.; School of Biotechnology and Biomolecular Science, UNSW Sydney, Kensington, NSW 2052, Australia.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2024 Jun 21; Vol. 10 (25), pp. eadk8501. Date of Electronic Publication: 2024 Jun 21.
DOI:	10.1126/sciadv.adk8501
Abstrakt:	Single-cell technology has allowed researchers to probe tissue complexity and dynamics at unprecedented depth in health and disease. However, the generation of high-dimensionality single-cell atlases and virtual three-dimensional tissues requires integrated reference maps that harmonize disparate experimental designs, analytical pipelines, and taxonomies. Here, we present a comprehensive single-cell transcriptome integration map of cardiac fibrosis, which underpins pathophysiology in most cardiovascular diseases. Our findings reveal similarity between cardiac fibroblast (CF) identities and dynamics in ischemic versus pressure overload models of cardiomyopathy. We also describe timelines for commitment of activated CFs to proliferation and myofibrogenesis, profibrotic and antifibrotic polarization of myofibroblasts and matrifibrocytes, and CF conservation across mouse and human healthy and diseased hearts. These insights have the potential to inform knowledge-based therapies.
Databáze:	MEDLINE
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