Near-infrared nanosensors enable optical imaging of oxytocin with selectivity over vasopressin in acute mouse brain slices.

Autor:	Mun J; California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720., Navarro N; Department of Chemistry, University of California, Berkeley, CA 94720., Jeong S; School of Biomedical Convergence Engineering, Pusan National University, Yangsan 50612, South Korea., Ouassil N; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720., Leem E; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720., Beyene AG; Janelia Research Campus, HHMI, Ashburn, VA 20147., Landry MP; California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720.; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720.; Chan Zuckerberg Biohub, San Francisco, CA 94158.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jun 25; Vol. 121 (26), pp. e2314795121. Date of Electronic Publication: 2024 Jun 21.
DOI:	10.1073/pnas.2314795121
Abstrakt:	Oxytocin plays a critical role in regulating social behaviors, yet our understanding of its function in both neurological health and disease remains incomplete. Real-time oxytocin imaging probes with spatiotemporal resolution relevant to its endogenous signaling are required to fully elucidate oxytocin's role in the brain. Herein, we describe a near-infrared oxytocin nanosensor (nIROXT), a synthetic probe capable of imaging oxytocin in the brain without interference from its structural analogue, vasopressin. nIROXT leverages the inherent tissue-transparent fluorescence of single-walled carbon nanotubes (SWCNT) and the molecular recognition capacity of an oxytocin receptor peptide fragment to selectively and reversibly image oxytocin. We employ these nanosensors to monitor electrically stimulated oxytocin release in brain tissue, revealing oxytocin release sites with a median size of 3 µm in the paraventricular nucleus of C57BL/6 mice, which putatively represents the spatial diffusion of oxytocin from its point of release. These data demonstrate that covalent SWCNT constructs, such as nIROXT, are powerful optical tools that can be leveraged to measure neuropeptide release in brain tissue. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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