Reshaping the Primary Cell Wall: Dual Effects on Plant Resistance to Ralstonia solanacearum and Heat Stress Response.

Autor: Desaint H; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan 31320, France.; SYNGENTA Seeds, Sarrians 84260, France., Gigli A; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan 31320, France.; Department of Biology, University of Florence, Sesto Fiorentino, Italy., Belny A; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan 31320, France., Cassan-Wang H; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse III, CNRS, INP, UMR5546, Castanet-Tolosan 31320, France., Martinez Y; Plateforme Imagerie, FRAIB-CNRS, Castanet-Tolosan 31320, France., Vailleau F; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan 31320, France., Mounet F; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse III, CNRS, INP, UMR5546, Castanet-Tolosan 31320, France., Vernhettes S; AgroParisTech, Institut Jean-Pierre Bourgin (IJPB), Université Paris-Saclay, INRAE, Versailles 78000, France., Berthomé R; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan 31320, France., Marchetti M; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, Castanet-Tolosan 31320, France.
Jazyk: angličtina
Zdroj: Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2024 Aug; Vol. 37 (8), pp. 619-634. Date of Electronic Publication: 2024 Aug 23.
DOI: 10.1094/MPMI-05-24-0059-R
Abstrakt: Temperature elevation drastically affects plant defense responses to Ralstonia solanacearum and inhibits the major source of resistance in Arabidopsis thaliana , which is mediated by the receptor pair RRS1-R/RPS4. In this study, we refined a previous genome-wide association (GWA) mapping analysis by using a local score approach and detected the primary cell wall CESA3 gene as a major gene involved in plant response to  R. solanacearum  at both 27°C and an elevated temperature, 30°C. We functionally validated  CESA3  as a susceptibility gene involved in resistance to  R. solanacearum  at both 27 and 30°C through a reverse genetic approach. We provide evidence that the  cesa3 mre1  mutant enhances resistance to bacterial disease and that resistance is associated with an alteration of root cell morphology conserved at elevated temperatures. However, even by forcing the entry of the bacterium to bypass the primary cell wall barrier, the  cesa3 mre1  mutant still showed enhanced resistance to  R. solanacearum  with delayed onset of bacterial wilt symptoms. We demonstrated that the  cesa3 mre1   mutant had constitutive expression of the defense-related gene  VSP1 , which is upregulated at elevated temperatures, and that during infection, its expression level is maintained higher than in the wild-type Col-0. In conclusion, this study reveals that alteration of the primary cell wall by mutating the cellulose synthase subunit CESA3 contributes to enhanced resistance to R. solanacearum , remaining effective under heat stress. We expect that these results will help to identify robust genetic sources of resistance to  R. solanacearum  in the context of global warming. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Competing Interests: The author(s) declare no conflict of interest.
Databáze: MEDLINE
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