Antibacterial activity of gallic acid and methyl gallate against emerging non-fermenting bacilli.

Autor: Flores-Maldonado O; Departamento de Microbiología, Facultad de Medicina y Hospital Universitario 'Dr, José Eleuterio González', Universidad Autónoma de Nuevo León, Av. Francisco I. Madero, Mitras Centro, 64460, Monterrey, Mexico., Dávila-Aviña J; Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, Mexico., González GM; Departamento de Microbiología, Facultad de Medicina y Hospital Universitario 'Dr, José Eleuterio González', Universidad Autónoma de Nuevo León, Av. Francisco I. Madero, Mitras Centro, 64460, Monterrey, Mexico., Becerril-García MA; Departamento de Microbiología, Facultad de Medicina y Hospital Universitario 'Dr, José Eleuterio González', Universidad Autónoma de Nuevo León, Av. Francisco I. Madero, Mitras Centro, 64460, Monterrey, Mexico., Ríos-López AL; Departamento de Microbiología, Facultad de Medicina y Hospital Universitario 'Dr, José Eleuterio González', Universidad Autónoma de Nuevo León, Av. Francisco I. Madero, Mitras Centro, 64460, Monterrey, Mexico. anarilopez@hotmail.com.
Jazyk: angličtina
Zdroj: Folia microbiologica [Folia Microbiol (Praha)] 2024 Jun 21. Date of Electronic Publication: 2024 Jun 21.
DOI: 10.1007/s12223-024-01182-z
Abstrakt: Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Burkholderia cenocepacia are considered emerging pathogens classified as a public health problem due to extensive antimicrobial resistance. Therefore, the discovery of new therapeutic strategies has become crucial. This study aimed to evaluate the antimicrobial activity of gallic acid and methyl gallate against non-fermenting bacteria. The study included five clinical isolates of Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Burkholderia cenocepacia. The minimum inhibitory concentrations of gallic acid and methyl gallate were determined by the broth microdilution method. Growth curves, metabolic activity, and biofilm formation of each bacterial strain in the presence or absence of phenolic compounds were performed. Finally, the therapeutic efficacy of the compounds was evaluated using an in vivo model. Gallic acid and methyl gallate showed antibacterial activity against bacterial strains in a concentration range of 64 to 256 µg/mL, both compounds reduced bacterial growth and metabolic activity of the strains, even at subinhibitory concentrations. Only, methyl gallate exhibited activity to inhibit the formation of bacterial biofilms. Moreover, gallic acid and methyl gallate increased larval survival by up to 60% compared to 30% survival of untreated larvae in a bacterial infection model in Galleria mellonella. Our results highlight the potential of gallic acid and methyl gallate as therapeutic alternatives for infections by emerging non-fermentative bacteria.
(© 2024. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.)
Databáze: MEDLINE
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