Label-free electrochemical aptasensor for the detection of HepG2 hepatocellular carcinoma cells.

Autor: Pusta A; Department of Analytical Chemistry, Iuliu Hațieganu' University of Medicine and Pharmacy, 4 Pasteur Street, Cluj-Napoca, 400349, Romania.; Department of Medical Devices, Iuliu Hațieganu' University of Medicine and Pharmacy, 4 Pasteur Street, Cluj-Napoca, 400349, Romania., Tertis M; Department of Analytical Chemistry, Iuliu Hațieganu' University of Medicine and Pharmacy, 4 Pasteur Street, Cluj-Napoca, 400349, Romania. mihaela.tertis@umfcluj.ro., Kezan D; Department of Analytical Chemistry, Iuliu Hațieganu' University of Medicine and Pharmacy, 4 Pasteur Street, Cluj-Napoca, 400349, Romania., Bogdan D; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath Street, Cluj-Napoca, 400293, Romania., Suciu M; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath Street, Cluj-Napoca, 400293, Romania.; Electron Microscopy Centre 'C. Craciun', Biology and Geology Faculty, Babes-Bolyai University Cluj-Napoca, 5- 7 Clinicilor Str., Romania, 400006 , Cluj-Napoca., Pană O; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath Street, Cluj-Napoca, 400293, Romania., Fizeșan I; Department of Toxicology, Iuliu Hațieganu' University of Medicine and Pharmacy, 8 Victor Babeș, Cluj- Napoca, 400012, Romania., Graur F; Department of Surgery 3, Iuliu Hațieganu' University of Medicine and Pharmacy, Croitorilor, Cluj- Napoca, 19-21, 400162, Romania., Cristea C; Department of Analytical Chemistry, Iuliu Hațieganu' University of Medicine and Pharmacy, 4 Pasteur Street, Cluj-Napoca, 400349, Romania. ccristea@umfcluj.ro., Al-Hajjar N; Department of Surgery 3, Iuliu Hațieganu' University of Medicine and Pharmacy, Croitorilor, Cluj- Napoca, 19-21, 400162, Romania.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Jun 21; Vol. 191 (7), pp. 413. Date of Electronic Publication: 2024 Jun 21.
DOI: 10.1007/s00604-024-06479-x
Abstrakt: Hepatocellular carcinoma (HCC) is the most common liver malignancy and is characterized by increasing incidence and high mortality rates. Current methods for the screening and diagnosis of HCC exhibit inherent limitations, highlighting the ever-growing need for the development of new methods for the early diagnosis of HCC. The aim of this work was to develop a novel electrochemical aptasensor for the detection of HepG2 cells, a type of circulating tumor cells that can be used as biomarkers for the early detection of HCC. A carbon screen-printed electrode was functionalized with a composite suspension containing graphene oxide, chitosan, and polyaniline nanoparticles to increase the electrode surface and provide anchoring sites for the HepG2 cell-specific aptamer. The aptamer was immobilized on the surface of the functionalized electrode using multipulse amperometry, an innovative technique that significantly reduces the time required for aptamer immobilization. The innovative platform was successfully employed for the first time for the amplification-free detection of HepG2 cells in a linear range from 10 to 200,000 cells/mL, with a limit of detection of 10 cells/mL. The platform demonstrated high selectivity and stability and was successfully used for the detection of HepG2 cells in spiked human serum samples with excellent recoveries.
(© 2024. The Author(s).)
Databáze: MEDLINE
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