Mesenchymal Stromal Cells Nanovesicles Carry microRNA with Nephroprotective Proprieties Regardless of Aging.

Autor:	Convento MB; Nephrology Division, Department of Medicine, Federal University of Sao Paulo, Street Borges Lagoa, 783, 6º Floor, Doctors' Building, Sao Paulo - SP - CEP 04038-901, Brazil., de Oliveira AS; Nephrology Division, Department of Medicine, Federal University of Sao Paulo, Street Borges Lagoa, 783, 6º Floor, Doctors' Building, Sao Paulo - SP - CEP 04038-901, Brazil., Boim MA; Nephrology Division, Department of Medicine, Federal University of Sao Paulo, Street Borges Lagoa, 783, 6º Floor, Doctors' Building, Sao Paulo - SP - CEP 04038-901, Brazil., Borges FT; Nephrology Division, Department of Medicine, Federal University of Sao Paulo, Street Borges Lagoa, 783, 6º Floor, Doctors' Building, Sao Paulo - SP - CEP 04038-901, Brazil.; Interdisciplinary Postgraduate Program in Health Sciences, Cruzeiro do Sul University, Street Galvão Bueno, 868 - Sao Paulo - SP, 01506-000, Brazil.
Jazyk:	angličtina
Zdroj:	Current aging science [Curr Aging Sci] 2024; Vol. 17 (2), pp. 118-126.
DOI:	10.2174/0118746098272926231107061047
Abstrakt:	Containing information molecules from their parent cells and inclining to fuse with targeted cells, bone marrow mesenchymal stromal cells-derived extracellular vesicles (MSCs- EV) are valuable in nanomedicine. Background: The effects of aging on the paracrine mechanism and in the production and action of MSCs-EV and their cargos of miR-26a and siRNA-26a for the treatment of tubular renal cells under nephrotoxicity injury remain unelucidated. Objective: The purpose of this study was to evaluate MSCs-EV of different ages and their ability to deliver the cargos of miR-26a and siRNA-26a to target renal tubular cells affected by nephrotoxicity injury. Methods: In a model of gentamicin-induced nephrotoxicity, renal tubular cells treated with MSCs-EV expressing or not expressing microRNA-26a were analyzed. Western blotting was utilized to evaluate cell cycle markers, and MTT assay was utilized to evaluate auto-renovation capacity. Results: Tubular cells under nephrotoxicity injury showed decreased proliferative capacity, but the treatment in the tubular renal cells under nephrotoxicity injury with MSCs-EV expressing microRNA-26a showed nephroprotective effects, regardless of EV age. While the treatment with EV-mediated siRNA-26a failed to preserve the nephroprotective effects equally, regardless of age. Conclusion: Mesenchymal stromal cell nanovesicles carry microRNA with nephroprotective proprieties regardless of aging. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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