Effect of photobiomodulation in the balance between effector and regulatory T cells in an experimental model of COPD.

Autor:	de Brito AA; Universidade Nove de Julho, São Paulo, Brazil.; Departament of Research Development and Innovation, Innovative Health System Health Management (HIS Medicine and Technology), São Paulo, Brazil., Herculano KZ; Departament of Research Development and Innovation, Innovative Health System Health Management (HIS Medicine and Technology), São Paulo, Brazil.; Departament of Surgery, Faculty of Veterinary, University of São Paulo, São Paulo, Brazil., de Alvarenga-Nascimento CR; Departament of Research Development and Innovation, Innovative Health System Health Management (HIS Medicine and Technology), São Paulo, Brazil., Estefano-Alves C; Universidade Nove de Julho, São Paulo, Brazil., Duran CCG; Universidade Nove de Julho, São Paulo, Brazil., Marcos RL; Universidade Nove de Julho, São Paulo, Brazil., Silva Junior JA; Universidade Nove de Julho, São Paulo, Brazil., Chavantes MC; Universidade Nove de Julho, São Paulo, Brazil., Zamuner SR; Universidade Nove de Julho, São Paulo, Brazil., Aimbire F; Translational Medicine, Federal University of São Paulo-UNIFESP, São José dos Campos, Brazil., Lladó-Pelfort L; Department of Basic Sciences, Faculty of Health Sciences at Manresa, University of Vic-Central University of Catalonia (UVic-UCC), Manresa, Spain., Gubern A; Department of Basic Sciences, Faculty of Health Sciences at Manresa, University of Vic-Central University of Catalonia (UVic-UCC), Manresa, Spain.; Faculty of Medicine, University of Vic-Central, Manresa, Spain., Fàbrega A; Department of Basic Sciences, Faculty of Health Sciences at Manresa, University of Vic-Central University of Catalonia (UVic-UCC), Manresa, Spain.; Faculty of Medicine, University of Vic-Central, Manresa, Spain.; Tissue Repair and Regeneration Laboratory (TR2Lab), Institute for Research and Innovation in Life and Health Sciences in Central Catalonia (Iris-CC), Vic, Spain., da Palma RK; Departament of Surgery, Faculty of Veterinary, University of São Paulo, São Paulo, Brazil.; Tissue Repair and Regeneration Laboratory (TR2Lab), Institute for Research and Innovation in Life and Health Sciences in Central Catalonia (Iris-CC), Vic, Spain.; Faculty of Health Sciences at Manresa, University of Vic-Central University of Catalonia (UVic-UCC), Manresa, Spain.; University Center of Anápolis, Anápolis, Brazil., Ligeiro de Oliveira AP; Universidade Nove de Julho, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in medicine [Front Med (Lausanne)] 2024 Jun 05; Vol. 11, pp. 1347517. Date of Electronic Publication: 2024 Jun 05 (Print Publication: 2024).
DOI:	10.3389/fmed.2024.1347517
Abstrakt:	Introduction: Currently, Chronic Obstructive Pulmonary Disease (COPD) has a high impact on morbidity and mortality worldwide. The increase of CD4+, CD8+ cells expressing NF-κB, STAT4, IFN-γ and perforin are related to smoking habit, smoking history, airflow rate, obstruction and pulmonary emphysema. Furthermore, a deficiency in CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) may impair the normal function of the immune system and lead to respiratory immune disease. On the other hand, the anti-inflammatory cytokine IL-10, produced by Treg cells and macrophages, inhibits the synthesis of several pro-inflammatory cytokines that are expressed in COPD. Therefore, immunotherapeutic strategies, such as Photobiomodulation (PBM), aim to regulate the levels of cytokines, chemokines and transcription factors in COPD. Consequently, the objective of this study was to evaluate CD4 + STAT4 and CD4 + CD25 + Foxp3 + cells as well as the production of CD4 + IFN- γ and CD4 + CD25 + IL-10 in the lung after PBM therapy in a COPD mice model. Methods: We induced COPD in C57BL/6 mice through an orotracheal application of cigarette smoke extract. PMB treatment was applied for the entire 7 weeks and Bronchoalveolar lavage (BAL) and lungs were collected to study production of IFN- γ and IL-10 in the lung. After the last administration with cigarette smoke extract (end of 7 weeks), 24 h later, the animals were euthanized. One-way ANOVA followed by NewmanKeuls test were used for statistical analysis with significance levels adjusted to 5% ( p < 0.05). Results: This result showed that PBM improves COPD symptomatology, reducing the number of inflammatory cells (macrophages, neutrophils and lymphocytes), the levels of IFN-γ among others, and increased IL-10. We also observed a decrease of collagen, mucus, bronchoconstriction index, alveolar enlargement, CD4+, CD8+, CD4+STAT4+, and CD4+IFN-γ+ cells. In addition, in the treated group, we found an increase in CD4+CD25+Foxp3+ and CD4+IL-10+ T cells. Conclusion: This study suggests that PBM treatment could be applied as an immunotherapeutic strategy for COPD. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Brito, Herculano, de Alvarenga-Nascimento, Estefano-Alves, Duran, Marcos, Silva Junior, Chavantes, Zamuner, Aimbire, Lladó-Pelfort, Gubern, Fàbrega, da Palma and Ligeiro de Oliveira.)
Databáze:	MEDLINE
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