Molecular surveillance for dengue serotypes among the population living in Moyen-Ogooué province, Gabon; evidence of the presence of dengue serotype 1.

Autor: Bikangui R; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon. bikrod16@gmail.com.; École doctorale régionale d'Afrique centrale en Infectiologie tropicale, Franceville, Gabon. bikrod16@gmail.com., Parkouda S; Centre Hospitalier Régional George RAWIRI, Lambaréné, Gabon., More A; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon., Magossou Mbadinga MV; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon., Boussoukou IPM; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon., Ondo GN; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon., Nkoma AMM; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon., Adamou R; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon., Honkpehedji YJ; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon.; Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.; Fondation pour la Recherche Scientifique (FORS), Cotonou, Benin., Rossatanga EG; Centre Hospitalier Régional George RAWIRI, Lambaréné, Gabon., Ushijima Y; Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.; Division of Biomedical Science, Institute of Medicine, University of Tsukuba, Tsukuba, Japan., Abe H; Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.; Institute of Tropical Medicine, Vietnam Research Station, Nagasaki University, Nagasaki, Japan., Lell B; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon.; Division of Infectious Diseases and Tropical Medicine, Department of Medicine 1, Medical University of Vienna, Vienna, Austria., Dejon-Agobé JC; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon., Yasuda J; Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.; National Research Center for the Control and Prevention of Infectious Diseases (CCPID), Nagasaki University, Nagasaki, Japan.; Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan., Adegnika AA; Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon.; École doctorale régionale d'Afrique centrale en Infectiologie tropicale, Franceville, Gabon.; Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The Netherlands.; Fondation pour la Recherche Scientifique (FORS), Cotonou, Benin.; Institut für Tropenmedizin, Universität Tübingen, and German Center for Infection Research (DZIF), Tübingen, Germany.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2024 Jun 20; Vol. 21 (1), pp. 141. Date of Electronic Publication: 2024 Jun 20.
DOI: 10.1186/s12985-024-02406-x
Abstrakt: Background: Despite dengue virus (DENV) outbreak in Gabon a decade ago, less is known on the potential circulation of DENV serotypes in the country. Previous studies conducted in some areas of the country, are limited to hospital-based surveys which reported the presence of some cases of serotype 2 and 3 seven years ago and more recently the serotype 1. As further investigation, we extend the survey to the community of Moyen Ogooué region with the aim to assess the presence of the dengue virus serotypes, additionally to characterize chikungunya (CHIKV) infection and describe the symptomatology associated with infections.
Method: A cross-sectional survey was conducted from April 2020 to March 2021. The study included participants of both sexes and any age one year and above, with fever or history of fever in the past seven days until blood collection. Eligible volunteers were clinically examined, and blood sample was collected for the detection of DENV and CHIKV using RT-qPCR. Positive samples were selected for the target sequencing.
Results: A total of 579 volunteers were included. Their mean age (SD) was 20 (20) years with 55% of them being female. Four cases of DENV infection were diagnosed giving a prevalence of 0.7% (95%CI: 0.2-1.8) in our cohort while no case of CHIKV was detected. The common symptoms and signs presented by the DENV cases included fatigue, arthralgia myalgia, cough, and loss of appetite. DENV-1was the only virus detected by RT-qPCR.
Conclusion: Our results confirm the presence of active dengue infection in the region, particularly DENV-1, and could suggest the decline of DENV-2 and DENV-3. Continuous surveillance remains paramount to comprehensively describe the extent of dengue serotypes distribution in the Moyen-Ogooué region of Gabon.
(© 2024. The Author(s).)
Databáze: MEDLINE
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