Expression of L1 Cell Adhesion Molecule, a Nephronal Principal Cell Marker, in Nephrogenic Adenoma.

Autor:	Mannan R; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Wang X; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Mahapatra S; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Wang S; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Chinnaiyan AK; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Skala SL; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Rogel Cancer Center, Michigan Medicine, Ann Arbor, Michigan., Zhang Y; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan., McMurry LM; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Zelenka-Wang S; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Cao X; Michigan Center for Translational Pathology, Ann Arbor, Michigan; Howard Hughes Medical Institute, Ann Arbor, Michigan., Sangoi AR; Department of Pathology, School of Medicine, Stanford Medicine, California., Dadhania V; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan., Picken MM; Department of Pathology and Laboratory Medicine, Loyola University Medical Center, Maywood, Illinois., Menon S; Department of Pathology, Tata Memorial Hospital, Homi Bhabha National Institute, Mumbai, Maharashtra, India., Al-Ahmadie H; Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York., Chinnaiyan AM; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan; Rogel Cancer Center, Michigan Medicine, Ann Arbor, Michigan; Howard Hughes Medical Institute, Ann Arbor, Michigan; Department of Urology, University of Michigan Medical School, Ann Arbor, Michigan., Dhanasekaran SM; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan., Mehra R; Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan; Michigan Center for Translational Pathology, Ann Arbor, Michigan; Rogel Cancer Center, Michigan Medicine, Ann Arbor, Michigan. Electronic address: mrohit@med.umich.edu.
Jazyk:	angličtina
Zdroj:	Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc [Mod Pathol] 2024 Aug; Vol. 37 (8), pp. 100540. Date of Electronic Publication: 2024 Jun 18.
DOI:	10.1016/j.modpat.2024.100540
Abstrakt:	Nephrogenic adenoma (NA) is a benign, reactive lesion seen predominantly in the urinary bladder and often associated with antecedent inflammation, instrumentation, or an operative history. Its histopathologic diversity can create diagnostic dilemmas and pathologists use morphologic evaluation along with available immunohistochemical (IHC) markers to navigate these challenges. IHC assays currently do not designate or specify NA's potential putative cell of origin. Leveraging single-cell RNA-sequencing technology, we nominated a principal (P) cell-collecting duct marker, L1 cell adhesion molecule (L1CAM), as a potential biomarker for NA. IHC characterization revealed L1CAM to be positive in all 35 (100%) patient samples of NA; negative expression was seen in the benign urothelium, benign prostatic glands, urothelial carcinoma (UCA) in situ, prostatic adenocarcinoma, majority of high-grade UCA, and metastatic UCA. In the study, we also used single-cell RNA sequencing to nominate a novel compendium of biomarkers specific for the proximal tubule, loop of Henle, and distal tubule (DT) (including P and intercalated cells), which can be used to perform nephronal mapping using RNA in situ hybridization and IHC technology. Employing this technique on NA we found enrichment of both the P-cell marker L1CAM and, the proximal tubule type-A and -B cell markers, PDZKI1P1 and PIGR, respectively. The cell-type markers for the intercalated cell of DTs (LINC01187 and FOXI1), and the loop of Henle (UMOD and IRX5), were found to be uniformly absent in NA. Overall, our findings show that based on cell type-specific implications of L1CAM expression, the shared expression pattern of L1CAM between DT P cells and NA. L1CAM expression will be of potential value in assisting surgical pathologists toward a diagnosis of NA in challenging patient samples. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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