The new CFEM protein CgCsa required for Fe 3+ homeostasis regulates the growth, development, and pathogenicity of Colletotrichum gloeosporioides.

Autor: Liu S; Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China; Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China., Bu Z; Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China., Zhang X; Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China., Chen Y; Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China., Sun Q; Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China., Wu F; Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China., Guo S; Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China., Zhu Y; Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China. Electronic address: Yonghuaz@outlook.com., Tan X; Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Yuelushan Laboratory, Changsha 410128, China; LongPing Branch, College of Biology, Hunan University, Changsha 410125, China. Electronic address: tanxinqiu2008@163.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Aug; Vol. 274 (Pt 1), pp. 133216. Date of Electronic Publication: 2024 Jun 18.
DOI: 10.1016/j.ijbiomac.2024.133216
Abstrakt: Secreted common fungal extracellular membrane (CFEM) domain proteins have been implicated in multiple biological functions in fungi. However, it is still largely unknown whether the ferric iron (Fe 3+ ), as an important trace element, was involved with the biological function of CFEM proteins. In this study, a new CFEM protein CgCsa, with high expression levels at the early inoculation stage on peppers by Colletotrichum gloeosporioides was investigated. Deletion of the targeted gene CgCsa revealed multiple biological roles in hyphal growth restriction, highly reduced conidial yield, delayed conidial germination, abnormal appressorium with elongated bud tubes, and significantly reduced virulence of C. gloeosporioides. Moreover, in CgCsa mutants, the expression levels of four cell wall synthesis-related genes were downregulated, and cell membrane permeability and electrical conductivity were increased. Compared to the wild-type, the CgCsa mutants downregulated expressions of iron transport-related genes, in addition, its three-dimensional structure was capable binding with iron. Increase in the Fe 3+ concentration in the culture medium partially recovered the functions of ΔCgCsa mutant. This is probably the first report to show the association between CgCsa and iron homeostasis in C. gloeosporioides. The results suggest an alternative pathway for controlling plant fungal diseases by deplete their trace elements.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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