Evaluation of effects of Tempol on testicular ischemia/reperfusion injury.

Autor: Ganjiani V; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Meimandi-Parizi A; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. Electronic address: meimandi@shirazu.ac.ir., Ahmadi N; Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Sharifiyazdi H; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Divar MR; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Jazyk: angličtina
Zdroj: The American journal of emergency medicine [Am J Emerg Med] 2024 Aug; Vol. 82, pp. 107-116. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1016/j.ajem.2024.06.009
Abstrakt: Aim: Tempol, a synthetic antioxidant compound, has received significant attention for its potential therapeutic applications in recent years, especially against ischemia/reperfusion (I/R) injury. The aim of the present research was to assess the protective effects of Tempol on testicular I/R injury caused by testicular torsion and detorsion (T/D) in rats.
Methods: The subjects were divided into five groups: sham, testicular T/D, testicular T/D with Tempol treatment at 50 and 100 mg/kg, and healthy rats treated with Tempol at 100 mg/kg. Testicular torsion was induced by rotating the left testicles for 2 h, followed by detorsion for 24 h. Testicular tissues were evaluated for gene expression, oxidative stress markers, and histopathology, epididymal sperms were stained and analyzed, and blood serum samples were collected to measure the testosterone hormone.
Results: The results showed that testicular I/R caused a significant decrease in sperm velocity parameters, viability, and count, as well as an increase in abnormal sperms (p < 0.05). However, treatment with Tempol significantly improved these parameters (p < 0.05). Histopathological analysis revealed severe damage to the testicular tissues, but treatment with Tempol improved the structural integrity of the seminiferous tubules. Testicular I/R also resulted in increased oxidative stress index and decreased testosterone levels significantly (p < 0.05), but Tempol administration mitigated these effects significantly (p < 0.05). Furthermore, the expression of Bax and Bcl 2 , genes associated with apoptosis, were significantly altered by testicular I/R (p < 0.05), but Tempol prevented these changes significantly (p < 0.05).
Conclusion: These findings provide strong evidence that Tempol can effectively prevent testicular I/R injury.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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