Efficacy and action mechanisms of compound Shen Chan decoction on experimental models of atopic dermatitis.

Autor: Zhang L; Zhejiang Wanli University, Ningbo 315100, China., Chen N; Ningbo Hospital of Traditional Chinese Medicine, Ningbo 315010, China., Liao Y; Zhejiang Pharmaceutical University, Ningbo 315100, China., Kong Y; Wenzhou Medical University, Wenzhou 325035, China., Yang X; Wenzhou Medical University, Wenzhou 325035, China., Zhan M; Zhejiang Pharmaceutical University, Ningbo 315100, China., Xu W; Zhejiang Pharmaceutical University, Ningbo 315100, China., Wang Y; Zhejiang Pharmaceutical University, Ningbo 315100, China., Zhu S; Zhejiang Wanli University, Ningbo 315100, China; Ningbo First Hospital, Ningbo 315010, China., Hu Y; Zhejiang Wanli University, Ningbo 315100, China; Zhejiang Pharmaceutical University, Ningbo 315100, China; Wenzhou Medical University, Wenzhou 325035, China. Electronic address: pharmhawk@126.com.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2024 Aug 20; Vol. 137, pp. 112479. Date of Electronic Publication: 2024 Jun 18.
DOI: 10.1016/j.intimp.2024.112479
Abstrakt: Shen chan decoction (SCD) as a significant Traditional Chinese medicine (TCM) to treat atopic dermatitis (AD), but its mechanism of action has not been clarified, so we started the present study, first possible effects of SCD on AD were predicted using network pharmacology. Next, dinitrochlorobenzene was used to establish a mouse model of AD. After successful modelling, the SCD were administered intragastrically to treat the mice. Eventually, the KEGG pathway enrichment analysis indicated that SCD improved AD mainly through effects on inflammation and the gut microbiota. The experimental findings revealed that SCD treatment attenuated AD symptoms and downregulate the characteristic immune factors, namely IL-4, IL-6 and IgE. Moreover, it promoted a balance between Th1/Th2 cells. Furthermore, the itch signaling pathways involving H1R/PAR-2/TRPV1 were inhibited. The 16S rRNA sequencing results indicated that SCD administration influenced the Firmicutes/Bacteroidetes ratio at the phylum level by augmenting the relative proportions of Lactobacillaceae and Muribaculaceae at the family and genus levels, while decreasing the abundances of Lactococcus and Ruminococcus. These findings suggest that internal administration of SCD is an effective therapeutic approach for AD. We suggest that SCD may be an alternative therapy for the treatment of AD.Additionally, it could offer valuable insights into the pathogenesis of AD and the development of innovative therapeutic agents.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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