Diversity and evolution of nitric oxide reduction in bacteria and archaea.

Autor:	Murali R; Department of Biochemistry, University of Illinois, Urbana-Champaign, Urbana, IL 61801.; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.; School of Life Sciences, University of Nevada, Las Vegas, Las Vegas, NV 89154., Pace LA; Department of Biochemistry, University of Illinois, Urbana-Champaign, Urbana, IL 61801.; meliora.bio, Salt Lake City, UT 84103., Sanford RA; Department of Earth Science and Environmental Change, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Ward LM; Department of Geosciences, Smith College, Northampton, MA 01063.; Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125., Lynes MM; Department of Chemistry and Biochemistry, Thermal Biology Institute, Montana State University, Bozeman, MT 59717.; Center for Biofilm Enginering, Montana State University, Bozeman, MT 59717., Hatzenpichler R; Department of Chemistry and Biochemistry, Thermal Biology Institute, Montana State University, Bozeman, MT 59717.; Center for Biofilm Enginering, Montana State University, Bozeman, MT 59717.; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717., Lingappa UF; Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125.; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720., Fischer WW; Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125., Gennis RB; Department of Biochemistry, University of Illinois, Urbana-Champaign, Urbana, IL 61801., Hemp J; meliora.bio, Salt Lake City, UT 84103.; Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jun 25; Vol. 121 (26), pp. e2316422121. Date of Electronic Publication: 2024 Jun 20.
DOI:	10.1073/pnas.2316422121
Abstrakt:	Nitrous oxide is a potent greenhouse gas whose production is catalyzed by nitric oxide reductase (NOR) members of the heme-copper oxidoreductase (HCO) enzyme superfamily. We identified several previously uncharacterized HCO families, four of which (eNOR, sNOR, gNOR, and nNOR) appear to perform NO reduction. These families have novel active-site structures and several have conserved proton channels, suggesting that they might be able to couple NO reduction to energy conservation. We isolated and biochemically characterized a member of the eNOR family from the bacterium Rhodothermus marinus and found that it performs NO reduction. These recently identified NORs exhibited broad phylogenetic and environmental distributions, greatly expanding the diversity of microbes in nature capable of NO reduction. Phylogenetic analyses further demonstrated that NORs evolved multiple times independently from oxygen reductases, supporting the view that complete denitrification evolved after aerobic respiration. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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