Chemical Resolution of an Epitope Recognized by Blood Group Antibodies Capable of Binding Both A and B Red Blood Cells.

Autor: Obukhova P; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.; FSBI - National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov Ministry of Health care of the Russian Federation, Moscow, Russian Federation., Tyrtysh T; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation., Tsygankova S; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation., Paramonov A; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation., Gordeeva E; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation., Shilova N; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.; FSBI - National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. Kulakov Ministry of Health care of the Russian Federation, Moscow, Russian Federation., Lipatnikov A; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation., Sokolova M; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation., Henry S; Kode Biotech, Auckland, New Zealand., Salimov E; I. M. Sechenov First Moscow State Medical University of the Ministry of Health care of the Russian Federation (Sechenov University), Moscow, Russian Federation., Bovin N; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation., Ryzhov I; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2024 Sep 02; Vol. 25 (17), pp. e202400430. Date of Electronic Publication: 2024 Aug 14.
DOI: 10.1002/cbic.202400430
Abstrakt: The high specificity of human antibodies to blood group A and B antigens is impressive, especially when considering the structural difference between these antigens (tetrasaccharides) is a NHAc versus a hydroxyl group on the terminal monosaccharide residue. It is well established that in addition to anti-A and anti-B there is a third antibody, anti-A,B capable of recognizing both A and B antigens. To analyze this AB specificity, we synthesized a tetrasaccharide, where the NHAc of the A antigen was replaced with an NH 2 . This NH 2 group was then used to attach the glycan to an affinity resin, creating an AB epitope (AB ep ) adsorbent where the critical site for recognition by A and B antibodies was not accessible, while the rest of the (conformationally compact) tetrasaccharide remained accessible. Anti-AB ep antibodies were then isolated from blood group O donors and found to have expected A,B specificity against immobilized and red cell bound synthetic antigens, including AB ep , and were able to agglutinate both A and B red cells. The amount of these anti-AB ep (anti-A,B) antibodies found in the blood of group O donors was comparable to levels of anti-A and anti-B found in group B and A individuals. Using STD-NMR the location for the AB epitope on the tetrasaccharide was found.
(© 2024 Wiley-VCH GmbH.)
Databáze: MEDLINE
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