Isolation, Identification, Phytochemical Characterization, and In Vitro Anti-Inflammatory Property of an Endophytic Trametes versicolor  CL-1 Isolated from Rosa   roxburghii.

Autor: Li L; Guizhou Medical University, Natural Products Research Center of Guizhou Provice, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Xian T; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Yan Y; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Chen F; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Liang Q; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Li H; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Yang L; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Li J; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Deng T; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Pan X; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHILE., Ge L; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Gao M; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Yang J; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Peng M; Guizhou Medical University, Natural Products Research Center of Guizhou Province, Guizhou Science City, Baiyun District, Guiyang City, Guizhou Province, 550014, Guiyang, CHINA., Yang X; Guizhou Medical University, State Key Laboratory of Functions and Applications of Medicinal Plants, Baijin Road, 550014, Guiyang, CHINA.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2024 Jun 20, pp. e202400873. Date of Electronic Publication: 2024 Jun 20.
DOI: 10.1002/cbdv.202400873
Abstrakt: This study explores the anti-inflammatory potential of an endophytic fungus, Trametes versicolor CL-1, isolated from the fruit tissues of Rosa roxburghii. Morphological and molecular analyses confirmed the identity of CL-1. An ethyl acetate extract (CL-E) from its fermentation broth was subjected to UPLC-HRMS and GNPS molecular networking. The analysis revealed a diverse array of secondary metabolites, including 11 terpenes, 7 flavonoids, 10 cinnamic acid derivatives, 6 oligopeptides, and 9 fatty acids, as verified by LC-MS/MS. Notably, CL-E exhibited significant in vitro anti-inflammatory activity in RAW264.7 cells. Furthermore,  molecular docking studies predicted favorable binding interactions of key compounds 1 within CL-E with the NLRP3 inflammasome (PDB ID: 6NPY). These findings suggest T. versicolor CL-1 as a promising source of natural anti-inflammatory agents and unveil R. roxburghii as a potential reservoir for discovering novel bioactive metabolites.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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