Efficient biodegradation of chlorobenzene via monooxygenation pathways by Pandoraea sp. XJJ-1 with high potential for groundwater bioremediation.

Autor: Pan L; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control On Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China., Yuan B; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control On Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China., Li Q; State Environment Protection Engineering Center for Urban Soil Contamination Control and Remediation, Shanghai Academy of Environmental Sciences, Shanghai, 200233, China., Ouyang J; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control On Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China., Yang J; State Environment Protection Engineering Center for Urban Soil Contamination Control and Remediation, Shanghai Academy of Environmental Sciences, Shanghai, 200233, China., Zhou Y; Sinopec Fifth Construction Co., Ltd, Guangzhou, 510145, China., Cui C; State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control On Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China. cuichangzheng@ecust.edu.cn.; Institute of Pollution Control and Ecological Security, Shanghai, 200092, China. cuichangzheng@ecust.edu.cn.
Jazyk: angličtina
Zdroj: International microbiology : the official journal of the Spanish Society for Microbiology [Int Microbiol] 2024 Jun 20. Date of Electronic Publication: 2024 Jun 20.
DOI: 10.1007/s10123-024-00544-4
Abstrakt: Chlorobenzene (CB), extensively used in industrial processes, has emerged as a significant contaminant in soil and groundwater. The eco-friendly and cost-effective microbial remediation has been increasingly favored to address this environmental challenge. In this study, a degrading bacterium was isolated from CB-contaminated soil at a pesticide plant, identified as Pandoraea sp. XJJ-1 (CCTCC M 2021057). This strain completely degraded 100 mg·L -1 CB and showed extensive degradability across a range of pH (5.0-9.0), temperature (10-37 °C), and CB concentrations (100-600 mg·L -1 ). Notably, the degradation efficiency was 85.2% at 15 °C, and the strain could also degrade six other aromatic hydrocarbons, including benzene, toluene, ethylbenzene, and xylene (o-, m-, p-). The metabolic pathway of CB was inferred using ultraperformance liquid chromatography, gas chromatography-mass spectrometry, and genomic analysis. In strain XJJ-1, CB was metabolized to o-chlorophenol and 3-chloroxychol by CB monooxygenase, followed by ortho-cleavage by the action of 3-chlorocatechol 1,2-dioxygenase. Moreover, the presence of the chlorobenzene monooxygenation pathway metabolism in strain XJJ-1 is reported for the first time in Pandoraea. As a bacterium with low-temperature resistance and composite pollutant degradation capacity, strain XJJ-1 has the potential application prospects in the in-situ bioremediation of CB-contaminated sites.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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