Gut mycobiome alterations in obesity in geographically different regions.

Autor:	Zhan H; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China., Wan Y; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China., Sun Y; Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.; Yunnan Province Clinical Research Center for Digestive Diseases, Yunnan, China.; Yunnan Geriatric Medical Center, Kunming, Yunnan, China., Xu Z; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China., Zhang F; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China., Yang K; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China., Zhu W; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China., Cheung CP; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China.; Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China., Tang W; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China., Ng EK; Department of Surgery, The Chinese University of Hong Kong, Hong Kong SAR, China., Wong SK; Department of Surgery, The Chinese University of Hong Kong, Hong Kong SAR, China., Yeoh YK; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China., Kl Chan F; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Centre for Gut Microbiota Research, The Chinese University of Hong Kong, Hong Kong SAR, China., Miao Y; Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.; Yunnan Province Clinical Research Center for Digestive Diseases, Yunnan, China., Zuo T; Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China., Zeng Z; Department of Organ Transplantation Center, The First Affiliated Hospital of Kunming Medical University, Kunming, China., Ng SC; Microbiota I-Center (MagIC) Limited, Hong Kong SAR, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China.; Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.
Jazyk:	angličtina
Zdroj:	Gut microbes [Gut Microbes] 2024 Jan-Dec; Vol. 16 (1), pp. 2367297. Date of Electronic Publication: 2024 Jun 20.
DOI:	10.1080/19490976.2024.2367297
Abstrakt:	The gut fungi play important roles in human health and are involved in energy metabolism. This study aimed to examine gut mycobiome composition in obese subjects in two geographically different regions in China and to identify specific gut fungi associated with obesity. A total of 217 subjects from two regions with different urbanization levels [Hong Kong (HK): obese, n  = 59; lean, n = 59; Kunming (KM): obese, n  = 50; lean, n  = 49. Mean body mass index (BMI) for obesity = 33.7] were recruited. We performed deep shotgun metagenomic sequencing on fecal samples to compare gut mycobiome composition and trophic functions in lean and obese subjects across these two regions. The gut mycobiome of obese subjects in both HK and KM were altered compared to those of lean subjects, characterized by a decrease in the relative abundance of Nakaseomyces , Schizosaccharomyces pombe , Candida dubliniensis and an increase in the abundance of Lanchanceathermotolerans , Saccharomyces paradox , Parastagonospora nodorum and Myceliophthorathermophila . Reduced fungal - bacterial and fungal - fungal correlations as well as increased negative fungal-bacterial correlations were observed in the gut of obese subjects. Furthermore, the anti-obesity effect of fungus S. pombe was further validated using a mouse model. Supplementing high-fat diet-induced obese mice with the fungus for 12 weeks led to a significant reduction in body weight gain ( p  < 0.001), and an improvement in lipid and glucose metabolism compared to mice without intervention. In conclusion, the gut mycobiome composition and functionalities of obese subjects were altered. These data shed light on the potential of utilizing fungus-based therapeutics for the treatment of obesity. S. pombe may serve as a potential fungal probiotic in the prevention of diet-induced obesity and future human trials are needed.
Databáze:	MEDLINE
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