Bioluminescent aptamer-based microassay for detection of melanoma inhibitory activity protein (MIA).

Autor: Bashmakova EE; Institute of Biophysics, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036, Krasnoyarsk, Russia. jeyn_a@bk.ru., Kudryavtsev AN; Institute of Biophysics, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036, Krasnoyarsk, Russia. jeyn_a@bk.ru., Tupikin AE; Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090, Novosibirsk, Russia., Kabilov MR; Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090, Novosibirsk, Russia., Sokolov AE; Kirensky Institute of Physics, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036, Krasnoyarsk, Russia.; Siberian Federal University, 660041, Krasnoyarsk, Russia., Frank LA; Institute of Biophysics, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036, Krasnoyarsk, Russia. jeyn_a@bk.ru.; Siberian Federal University, 660041, Krasnoyarsk, Russia.
Jazyk: angličtina
Zdroj: Analytical methods : advancing methods and applications [Anal Methods] 2024 Jul 04; Vol. 16 (26), pp. 4234-4239. Date of Electronic Publication: 2024 Jul 04.
DOI: 10.1039/d4ay00706a
Abstrakt: Melanoma inhibitory activity protein (MIA) does obviously offer the potential to reveal clinical manifestations of melanoma. Despite a pressing need for effective diagnosis of this highly fatal disease, there are no clinically approved MIA detection ELISA kits available. A recommended MIA threshold has not yet been defined, mostly by reason of variability in immunoglobulins' affinity and stability, the difference in sample preparation and assay conditions. Here we present a pair of high-affinity DNA aptamers developed as an alternative recognition and binding element for MIA detection. Their stability and reproducible synthesis are expected to ensure this analysis under standard conditions. The devised aptamer-based solid-phase microassay of model standard and control human sera involves luciferase NLuc as a highly sensitive reporter. Bioluminescence dependence on MIA concentration ranges in a linear manner from 2.5 to 250 ng mL -1 , providing a MIA detection limit of 1.67 ± 0.57 ng mL -1 .
Databáze: MEDLINE
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