The first ITS1 profiling of honey samples from the Southeast Asian region Lombok, Bali and Banggi Island.

Autor: Ullah S; Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Johor Bahru, Malaysia., Huyop F; Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Johor Bahru, Malaysia. fahrul@utm.my.; Bioindustry Laboratory, Department of Agro-Industrial Technology, Udayana University, Denpasar, Indonesia. fahrul@utm.my., Wahab RA; Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310, Johor Bahru, Malaysia., Huda N; Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, 90509, Sandakan, Sabah, Malaysia., Oyewusi HA; Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Johor Bahru, Malaysia.; Biochemistry Unit, Department of Science Technology, The Federal Polytechnic, P. M. B 5351, Ado Ekiti, Ekiti State, Nigeria., Sujana IGA; Bioindustry Laboratory, Department of Agro-Industrial Technology, Udayana University, Denpasar, Indonesia., Saloko S; Faculty of Food Technology and Agro Industry, University of Mataram, Mataram, Nusa Tenggara Barat, 83126, Indonesia., Andriani AASPR; Faculty of Agriculture, Warmadewa University, Denpasar, Indonesia., Mohamad MAN; Research Unit for Bioinformatics and Computational Biology (RUBIC), Kulliyyah of Science, International Islamic University Malaysia, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia., Abdul Hamid AA; Research Unit for Bioinformatics and Computational Biology (RUBIC), Kulliyyah of Science, International Islamic University Malaysia, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia., Mohd Nasir MH; Research Unit for Bioinformatics and Computational Biology (RUBIC), Kulliyyah of Science, International Islamic University Malaysia, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia., Antara NS; Bioindustry Laboratory, Department of Agro-Industrial Technology, Udayana University, Denpasar, Indonesia., Gunam IBW; Bioindustry Laboratory, Department of Agro-Industrial Technology, Udayana University, Denpasar, Indonesia. ibwgunam@unud.ac.id.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jun 19; Vol. 14 (1), pp. 14122. Date of Electronic Publication: 2024 Jun 19.
DOI: 10.1038/s41598-024-64838-3
Abstrakt: Southern Asian flowers offer honeybees a diversity of nectar. Based on its geographical origin, honey quality varies. Traditional methods are less authentic than DNA-based identification. The origin of honey is determined by pollen, polyphenolic, and macro-microorganisms. In this study, amplicon sequencing targets macro-microorganisms in eDNA using the ITS1 region to explore honey's geographical location and authentication. The variety of honey samples was investigated using ITS1 with Illumina sequencing. For all four honey samples, raw sequence reads showed 979,380 raw ITS1 amplicon reads and 375 ASVs up to the phylum level. The highest total number of 202 ASVs up to phylum level identified Bali honey with 211,189 reads, followed by Banggi honey with 309,207 a total number of 111 ASVs, and Lombok represents only 63 ASVs up to phylum level with several read 458,984. Based on Shannon and Chao1, honey samples from Bali (B2) and (B3) exhibited higher diversity than honey from Lombok (B1) and green honey from Sabah (B4), while the Simpson index showed that Banggi honey (B4) had higher diversity. Honey samples had significant variance in mycobiome taxonomic composition and abundance. Zygosaccharomyces and Aspergillus were the main genera found in Lombok honey, with percentages of 68.81% and 29.76% respectively. Bali honey samples (B2 and B3) were identified as having a significant amount of the genus Aureobasidium, accounting for 40.81% and 25% of the readings, respectively. The microbiome composition of Banggi honey (B4) showed a high presence of Zygosaccharomyces 45.17% and Aureobasidium 35.24%. The ITS1 analysis effectively distinguishes between honey samples of different origins and its potential as a discriminatory tool for honey origin and authentication purposes.
(© 2024. The Author(s).)
Databáze: MEDLINE
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