High-throughput volumetric mapping of synaptic transmission.

Autor: Chen W; Department of Physics, University of California, Berkeley, CA, USA.; School of Mechanical Science and Engineering - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan, China., Ge X; Department of Physiology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA., Zhang Q; Department of Physics, University of California, Berkeley, CA, USA., Natan RG; Department of Physics, University of California, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA., Fan JL; Joint Bioengineering Graduate Program, University of California, Berkeley, CA, USA.; Joint Bioengineering Graduate Program, University of California, San Francisco, CA, USA., Scanziani M; Department of Physiology, University of California, San Francisco, San Francisco, CA, USA.; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA., Ji N; Department of Physics, University of California, Berkeley, CA, USA. jina@berkeley.edu.; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. jina@berkeley.edu.; Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA. jina@berkeley.edu.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. jina@berkeley.edu.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2024 Jul; Vol. 21 (7), pp. 1298-1305. Date of Electronic Publication: 2024 Jun 19.
DOI: 10.1038/s41592-024-02309-3
Abstrakt: Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at >1,000 synapses per volume and >500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.
(© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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