Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila.

Autor: D'Ermo G; Aix-Marseille Université, CNRS, BIP-UMR 7281, Marseille, France., Audebert S; Aix-Marseille Université, Inserm, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Protéomique, Marseille, France., Camoin L; Aix-Marseille Université, Inserm, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Protéomique, Marseille, France., Planer-Friedrich B; Environmental Geochemistry, Bayreuth Centre for Ecology and Environmental Research (BAYCEER), University of Bayreuth, Bayreuth, Germany., Casiot-Marouani C; Laboratoire HydroSciences Montpellier, Univ. Montpellier, CNRS, IRD, Montpellier, France., Delpoux S; Laboratoire HydroSciences Montpellier, Univ. Montpellier, CNRS, IRD, Montpellier, France., Lebrun R; Aix-Marseille Université, CNRS, IMM-FR3479, Marseille Protéomique, Marseille, France., Guiral M; Aix-Marseille Université, CNRS, BIP-UMR 7281, Marseille, France., Schoepp-Cothenet B; Aix-Marseille Université, CNRS, BIP-UMR 7281, Marseille, France.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2024 Jun; Vol. 26 (6), pp. e16655.
DOI: 10.1111/1462-2920.16655
Abstrakt: The metabolic process of purple sulphur bacteria's anoxygenic photosynthesis has been primarily studied in Allochromatium vinosum, a member of the Chromatiaceae family. However, the metabolic processes of purple sulphur bacteria from the Ectothiorhodospiraceae and Halorhodospiraceae families remain unexplored. We have analysed the proteome of Halorhodospira halophila, a member of the Halorhodospiraceae family, which was cultivated with various sulphur compounds. This analysis allowed us to reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the Ectothiorhodospiraceae family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of Halorhodospira halophila when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bacteria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron carriers, cytochrome c 551 /c 5 and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome c 551 /c 5 and HiPIP III directing electrons towards the Reaction Centre.
(© 2024 The Author(s). Environmental Microbiology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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