A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative.

Autor: Dos Santos CWV; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Postal Code: 57072-900, Maceió, Alagoas, Brazil., Da Silva AT; Institute of Pharmaceutical Science, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Postal Code: 57072-900, Maceió, Alagoas, Brazil., de Almeida Barros AC; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Postal Code: 57072-900, Maceió, Alagoas, Brazil., do Nascimento JS; Institute of Pharmaceutical Science, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Postal Code: 57072-900, Maceió, Alagoas, Brazil., Meireles Grillo LA; Institute of Pharmaceutical Science, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Postal Code: 57072-900, Maceió, Alagoas, Brazil., Gomes FS; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Postal Code: 57072-900, Maceió, Alagoas, Brazil., Pereira HJV; Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Postal Code: 57072-900, Maceió, Alagoas, Brazil. Electronic address: hugo.vieira@iqb.ufal.br.
Jazyk: angličtina
Zdroj: Protein expression and purification [Protein Expr Purif] 2024 Oct; Vol. 222, pp. 106534. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1016/j.pep.2024.106534
Abstrakt: Tribolium castaneum, also known as the red flour beetle, is a polyphagous pest that seriously damages agricultural products, including stored and processed grains. Researchers have aimed to discover alternative pest control mechanisms that are less harmful to the ecosystem than those currently used. We conduct the purification and characterization of a protease inhibitor from C. plumieri seeds and an in vitro evaluation of its insecticidal potential against the insect pest T. castaneum. The trypsin inhibitor was isolated from C. plumieri seeds in a single-step DEAE-Sepharose column chromatography and had a molecular mass of 50 kDA. When analyzed for interaction with different proteolytic enzymes, the inhibitor exhibited specificity against trypsin and no activity against other serine proteases such as chymotrypsin and elastase-2. The isolated inhibitor was able to inhibit digestive enzymes of T. castaneum from extracts of the intestine of this insect. Therefore, we conclude that the new protease inhibitor, specific in tryptic inhibition, of protein nature from the seeds of C. plumieri was effective in inhibiting the digestive enzymes of T. castaneum and is a promising candidate in the ecological control of pests.
Competing Interests: Declaration of competing interest The authors whose names are listed immediately below certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: