Antibiofilm and antivirulence activity of selenium nanoparticles synthesized from cell-free extract of moderately halophilic bacteria.

Autor: Annamalai KK; Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, 600119, Tamil Nadu, India., Selvaraj B; Department of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600007, Tamil Nadu, India. Electronic address: sbharathimicro@gmail.com., Subramanian K; PG and Research Department of Microbiology, Sri Sankara Arts and Science College, Kancheepuram, 631561, Tamil Nadu, India., Binsuwaidan R; Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O.Box 84428, Riyadh 11671, Saudi Arabia., Saeed M; Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Aug; Vol. 193, pp. 106740. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1016/j.micpath.2024.106740
Abstrakt: Biofilm-forming microbes can pose a major health risk that is difficult to combat. Nanotechnology, on the other hand, represents a novel technique for combating and eliminating biofilm-forming microbes. In this study, the selenium nanoparticles (SeNPs) were biosynthesized from moderate halophilic bacteria isolated from Pichavaram mangrove sediments. The bacterial strain S8 was found to be efficient for SeNPs synthesis and hence identified by 16s r RNA sequencing as Shewanella sp. In UV- spectral analysis the SeNPs displayed a peak at 320 nm due to surface plasmon resonance (SPR). The cell-free extract of Shewanella sp. and SeNPs indicates that the various functional groups in the cell-free extract were mainly involved in the synthesis and stabilization of SeNPs. The SeNPs had a spherical form with average diameter of 49 ± 0.01 nm, according to the FESEM analysis. The EDX shows the distinctive peaks of selenium at 1.37, 11.22.12.49 Kev. In the agar well diffusion method, the SeNPs show inhibitory activity against all the test pathogens with the highest activity noted against P.aeruginosa with a zone of inhibition of 22.7 ± 0.3 mm. The minimal inhibitory concentration (MIC) value of 80 μg/ml, minimal bactericidal concentration (MBC) of 160 μg/ml, and susceptibility constant of 0.043 μg/ml show that SeNPs highly effective against P.aeruginosa. The Sub-MIC value of SeNPs of 20 μg/ml was found to inhibit P.aeruginosa biofilm by 85% as compared to the control. Further, the anti-virulence properties viz., pyocyanin, pyoverdine, hemolytic, and protease inhibition revealed the synthesized SeNPs from halophilic bacteria control the pathogenicity of P.aeruginosa.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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