An anti-mitotic compound, (+)-6-tuliposide A, isolated from the Canadian glacier lily, Erythronium grandiflorum.

Autor: Healy Knibb SM; Natural Product Laboratory, University of Lethbridge, 4401 University Drive W, Lethbridge, AB T1K 3M4, Canada., Yeremy B; Departments of Chemistry and EOAS, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z6, Canada., Williams DE; Departments of Chemistry and EOAS, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z6, Canada., Andersen RJ; Departments of Chemistry and EOAS, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z6, Canada., Golsteyn RM; Natural Product Laboratory, University of Lethbridge, 4401 University Drive W, Lethbridge, AB T1K 3M4, Canada. Electronic address: roy.golsteyn@uleth.ca.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2024 Sep; Vol. 177, pp. 106075. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1016/j.fitote.2024.106075
Abstrakt: The Canadian prairie ecosystem is subjected to abiotic and biotic conditions that induce plants to produce secondary metabolites that affect mammalian physiology. Extracts prepared from certain plant species native to Canadian prairie and montane cordillera ecosystems have previously been shown to have anti-mitotic activity on human cancer cell lines. In this study, we investigated the glacier lily, Erythronium grandiflorum (Liliaceae), in which the species was the most phylogenetically distant from Asteraceae and had anti-mitotic activity. When added to cell lines, E. grandiflorum extracts induced rounded cell morphology and arrested cells in the G2/M phase of the cell cycle. Of the cells that displayed a rounded phenotype, all were positive for phospho-histone H3 and contained a distorted mitotic spindle. This anti-mitotic activity was distinct from that of the compound colchicine, which has been previously isolated from the Liliaceae family. By biology-guided fractionation, we isolated the natural product (+)-6-tuliposide A and are the first to report its anti-mitotic activity. These results reveal a chemical motif in secondary metabolites and expand the range of Canadian prairie plants with anti-mitotic activity that can become new scientific tools or used in the development of anti-proliferative medicines.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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