3D electronic implants in subretinal space: Long-term follow-up in rodents.

Autor: Bhuckory MB; Department of Ophthalmology, Stanford University, Stanford, CA, USA; Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, USA. Electronic address: bhuckory@stanford.edu., Wang BY; Department of Physics, Stanford University, Stanford, CA, USA., Chen ZC; Department of Electrical Engineering, Stanford University, Stanford, CA, USA., Shin A; Department of Material Science, Stanford University, Stanford, CA, USA., Pham-Howard D; Department of Ophthalmology, Stanford University, Stanford, CA, USA; Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, USA., Shah S; Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, USA., Monkongpitukkul N; Department of Ophthalmology, Stanford University, Stanford, CA, USA; Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, USA., Galambos L; Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, USA., Kamins T; Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, USA; Department of Electrical Engineering, Stanford University, Stanford, CA, USA., Mathieson K; Department of Physics, Institute of Photonics, University of Strathclyde, Glasgow, Scotland, UK., Palanker D; Department of Ophthalmology, Stanford University, Stanford, CA, USA; Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA, USA.
Jazyk: angličtina
Zdroj: Biomaterials [Biomaterials] 2024 Dec; Vol. 311, pp. 122674. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1016/j.biomaterials.2024.122674
Abstrakt: Clinical results with photovoltaic subretinal prosthesis (PRIMA) demonstrated restoration of sight via electrical stimulation of the interneurons in degenerated retina, with resolution matching the 100 μm pixel size. Since scaling the pixels below 75 μm in the current bipolar planar geometry will significantly limit the penetration depth of the electric field and increase stimulation threshold, we explore the possibility of using smaller pixels based on a novel 3-dimensional honeycomb-shaped design. We assessed the long-term biocompatibility and stability of these arrays in rats by investigating the anatomical integration of the retina with flat and 3D implants and response to electrical stimulation over lifetime - up to 32-36 weeks post-implantation in aged rats. With both flat and 3D implants, signals elicited in the visual cortex decreased after the day of implantation by more than 3-fold, and gradually recovered over the next 12-16 weeks. With 25 μm high honeycomb walls, the majority of bipolar cells migrate into the wells, while amacrine and ganglion cells remain above the cavities, which is essential for selective network-mediated stimulation of the retina. Retinal thickness and full-field stimulation threshold with 40 μm-wide honeycomb pixels were comparable to those with planar devices - 0.05 mW/mm 2 with 10 ms pulses. However, fewer cells from the inner nuclear layer migrated into the 20 μm-wide wells, and stimulation threshold increased over 12-16 weeks, before stabilizing at about 0.08 mW/mm 2 . Such threshold is still significantly lower than 1.8 mW/mm 2 with a previous design of flat bipolar pixels, confirming the promise of the 3D honeycomb-based approach to high resolution subretinal prosthesis.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Daniel Palanker reports financial support was provided by National Institutes of Health. Daniel Palanker reports financial support was provided by U.S. Department of Defense. Daniel Palanker reports financial support was provided by Air Force Office of Scientific Research. Daniel Palanker reports a relationship with Pixium Vision that includes: consulting or advisory. Ted Kamins reports a relationship with Pixium Vision that includes: consulting or advisory. Daniel Palanker has patent licensed to Pixium Vision. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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