All-in-one platform: Versatile, Easy, and User-friendly System (VEUS) based on automated and expert-independent antibody immobilization and immunoassay by utilizing customized movement of magnetic particles.

Autor: Yoon J; Institute for Wearable Convergence Electronics, College of Electronics and Information, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea., Kim J; EzDiaTech Inc. Anyang, Gyeonggi, Republic of Korea., Lim S; Institute for Wearable Convergence Electronics, College of Electronics and Information, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea., Choi H; EzDiaTech Inc. Anyang, Gyeonggi, Republic of Korea., Bae J; Department of Electronics and Information Convergence Engineering, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea., Kim K; Department of Electronic Engineering, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea., Song SH; Institute for Wearable Convergence Electronics, College of Electronics and Information, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea., Cho YB; EzDiaTech Inc. Anyang, Gyeonggi, Republic of Korea., Park W; Institute for Wearable Convergence Electronics, College of Electronics and Information, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea.; Department of Electronics and Information Convergence Engineering, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea.; Department of Electronic Engineering, Kyung Hee University, Yongin, Gyeonggi, Republic of Korea., Jung YG; EzDiaTech Inc. Anyang, Gyeonggi, Republic of Korea.
Jazyk: angličtina
Zdroj: Biotechnology journal [Biotechnol J] 2024 Jun; Vol. 19 (6), pp. e2400074.
DOI: 10.1002/biot.202400074
Abstrakt: The ELISA is the most worldwide method for immunoassay. However, the ELISA is losing ground due to low reproducibility of manual experimental processes in both R&D and IVD areas. An automated platform is a good solution, but there are still limitations owning to extremely high cost and requiring large space to set up especially for a small size laboratory. Here, we present a novel all-in-one platform called "VEUS" settable on the laboratory table that offers comprehensive automation of the entire multiplex immunoassay process by exploiting antibody conjugated magnetic particles, quality control and then immunoanalytical reaction, thereby enhancing detection sensitivity and high reproducibility. As a proof of concept, the system exhibits a sensitive LOD of 0.6 and 3.1 pg mL -1 within 1 h run, comparable precision that of molecular diagnostic systems based on PCR method, enabling rapid multiplex diagnosis of Influenza A, Influenza B, and COVID-19 viruses with similar symptoms. Through automation by the all-in-one system, it can be used by novice users, something innovative for immunoassays, relying heavily on user experience. Furthermore, it can contribute to streamline entire immunoassay processes of diverse biomarkers with high reproducibility and convenience in laboratories.
(© 2024 The Authors. Biotechnology Journal published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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