Fiber-based Probes for Electrophysiology, Photometry, Optical and Electrical Stimulation, Drug Delivery, and Fast-Scan Cyclic Voltammetry In Vivo.

Autor:	Driscoll N; Massachusetts Institute of Technology, Cambridge, MA 02139., Antonini MJ; Massachusetts Institute of Technology, Cambridge, MA 02139., Cannon TM; Massachusetts Institute of Technology, Cambridge, MA 02139., Maretich P; Massachusetts Institute of Technology, Cambridge, MA 02139., Olaitan G; The University of Virginia, Charlottesville, VA 22904., Phi Van VD; Massachusetts Institute of Technology, Cambridge, MA 02139., Nagao K; Massachusetts Institute of Technology, Cambridge, MA 02139., Sahasrabudhe A; Massachusetts Institute of Technology, Cambridge, MA 02139., Vargas E; Massachusetts Institute of Technology, Cambridge, MA 02139., Hunt S; Stanford University, Stanford, CA 94305., Hummel M; Massachusetts Institute of Technology, Cambridge, MA 02139., Mupparaju S; Massachusetts Institute of Technology, Cambridge, MA 02139., Jasanoff A; Massachusetts Institute of Technology, Cambridge, MA 02139., Venton J; The University of Virginia, Charlottesville, VA 22904., Anikeeva P; Massachusetts Institute of Technology, Cambridge, MA 02139.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 08. Date of Electronic Publication: 2024 Jun 08.
DOI:	10.1101/2024.06.07.598004
Abstrakt:	Recording and modulation of neuronal activity enables the study of brain function in health and disease. While translational neuroscience relies on electrical recording and modulation techniques, mechanistic studies in rodent models leverage genetic precision of optical methods, such as optogenetics and imaging of fluorescent indicators. In addition to electrical signal transduction, neurons produce and receive diverse chemical signals which motivate tools to probe and modulate neurochemistry. Although the past decade has delivered a wealth of technologies for electrophysiology, optogenetics, chemical sensing, and optical recording, combining these modalities within a single platform remains challenging. This work leverages materials selection and convergence fiber drawing to permit neural recording, electrical stimulation, optogenetics, fiber photometry, drug and gene delivery, and voltammetric recording of neurotransmitters within individual fibers. Composed of polymers and non-magnetic carbon-based conductors, these fibers are compatible with magnetic resonance imaging, enabling concurrent stimulation and whole-brain monitoring. Their utility is demonstrated in studies of the mesolimbic reward pathway by simultaneously interfacing with the ventral tegmental area and nucleus accumbens in mice and characterizing the neurophysiological effects of a stimulant drug. This study highlights the potential of these fibers to probe electrical, optical, and chemical signaling across multiple brain regions in both mechanistic and translational studies. Competing Interests: Conflict of Interest N.D., M.-J. A. and P.A. are co-founders and have a financial interest in NeuroBionics Inc.
Databáze:	MEDLINE
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