Parallel on-chip micropipettes enabling quantitative multiplexed characterization of vesicle mechanics and cell aggregates rheology.

Autor:	Landiech S; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Elias M; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Lapèze P; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Ajiyel H; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Plancke M; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., González-Bermúdez B; Center for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, Spain and Department of Materials Science, ETSI de Caminos, Canales y Puertos, Universidad Politécnica de Madrid, Madrid, Spain., Laborde A; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Mesnilgrente F; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Bourrier D; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Berti D; CSGI and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy., Montis C; CSGI and Department of Chemistry, University of Florence, Sesto Fiorentino, Italy., Mazenq L; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Baldo J; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Roux C; SoftMat, Université de Toulouse, CNRS, UPS, Toulouse, France., Delarue M; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France., Joseph P; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France.
Jazyk:	angličtina
Zdroj:	APL bioengineering [APL Bioeng] 2024 Jun 12; Vol. 8 (2), pp. 026122. Date of Electronic Publication: 2024 Jun 12 (Print Publication: 2024).
DOI:	10.1063/5.0193333
Abstrakt:	Micropipette aspiration (MPA) is one of the gold standards for quantifying biological samples' mechanical properties, which are crucial from the cell membrane scale to the multicellular tissue. However, relying on the manipulation of individual home-made glass pipettes, MPA suffers from low throughput and no automation. Here, we introduce the sliding insert micropipette aspiration method, which permits parallelization and automation, thanks to the insertion of tubular pipettes, obtained by photolithography, within microfluidic channels. We show its application both at the lipid bilayer level, by probing vesicles to measure membrane bending and stretching moduli, and at the tissue level by quantifying the viscoelasticity of 3D cell aggregates. This approach opens the way to high-throughput, quantitative mechanical testing of many types of biological samples, from vesicles and individual cells to cell aggregates and explants, under dynamic physico-chemical stimuli. Competing Interests: The authors have no conflicts to disclose. (© 2024 Author(s).)
Databáze:	MEDLINE
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