A Prebiotic Diet Containing Galactooligosaccharides and Polydextrose Produces Dynamic and Reproducible Changes in the Gut Microbial Ecosystem in Male Rats.

Autor: Thompson RS; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, USA.; Center for Neuroscience, University of Colorado Boulder, Boulder, CO 80309, USA., Bowers SJ; Department of Neurobiology, Northwestern University, Center for Sleep and Circadian Biology, Evanston, IL 60208, USA., Vargas F; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, San Diego, CA 92093, USA., Hopkins S; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, USA.; Center for Neuroscience, University of Colorado Boulder, Boulder, CO 80309, USA., Kelley T; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, USA., Gonzalez A; Department of Pediatrics, University of California San Diego, San Diego, CA 92093, USA., Lowry CA; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, USA.; Center for Neuroscience, University of Colorado Boulder, Boulder, CO 80309, USA., Dorrestein PC; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, San Diego, CA 92093, USA., Vitaterna MH; Department of Neurobiology, Northwestern University, Center for Sleep and Circadian Biology, Evanston, IL 60208, USA., Turek FW; Department of Neurobiology, Northwestern University, Center for Sleep and Circadian Biology, Evanston, IL 60208, USA., Knight R; Department of Pediatrics, University of California San Diego, San Diego, CA 92093, USA.; Department of Computer Science and Engineering, University of California San Diego, San Diego, CA 92093, USA.; Center for Microbiome Innovation, University of California San Diego, San Diego, CA 92093, USA., Wright KP Jr; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, USA.; Center for Neuroscience, University of Colorado Boulder, Boulder, CO 80309, USA., Fleshner M; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, USA.; Center for Neuroscience, University of Colorado Boulder, Boulder, CO 80309, USA.
Jazyk: angličtina
Zdroj: Nutrients [Nutrients] 2024 Jun 06; Vol. 16 (11). Date of Electronic Publication: 2024 Jun 06.
DOI: 10.3390/nu16111790
Abstrakt: Despite substantial evidence supporting the efficacy of prebiotics for promoting host health and stress resilience, few experiments present evidence documenting the dynamic changes in microbial ecology and fecal microbially modified metabolites over time. Furthermore, the literature reports a lack of reproducible effects of prebiotics on specific bacteria and bacterial-modified metabolites. The current experiments examined whether consumption of diets enriched in prebiotics (galactooligosaccharides (GOS) and polydextrose (PDX)), compared to a control diet, would consistently impact the gut microbiome and microbially modified bile acids over time and between two research sites. Male Sprague Dawley rats were fed control or prebiotic diets for several weeks, and their gut microbiomes and metabolomes were examined using 16S rRNA gene sequencing and untargeted LC-MS/MS analysis. Dietary prebiotics altered the beta diversity, relative abundance of bacterial genera, and microbially modified bile acids over time. PICRUSt2 analyses identified four inferred functional metabolic pathways modified by the prebiotic diet. Correlational network analyses between inferred metabolic pathways and microbially modified bile acids revealed deoxycholic acid as a potential network hub. All these reported effects were consistent between the two research sites, supporting the conclusion that dietary prebiotics robustly changed the gut microbial ecosystem. Consistent with our previous work demonstrating that GOS/PDX reduces the negative impacts of stressor exposure, we propose that ingesting a diet enriched in prebiotics facilitates the development of a health-promoting gut microbial ecosystem.
Databáze: MEDLINE