| Autor: |
Kaur D; Department of Plant Science, McGill University, 21,111 rue Lakeshore, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada.; Institut de Recherche en Biologie Végétale, Université de Montréal, 4101 rue Sherbrooke E., Montréal, QC H1X 2B2, Canada., Schedl A; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 52, 04103 Leipzig, Germany.; Institute of Biodiversity, Friedrich Schiller University Jena, 07743 Jena, Germany.; German Biomass Research Centre (DBFZ), Torgauer Straße 116, 04347 Leipzig, Germany., Lafleur C; Department of Animal Science, McGill University, 21,111 rue Lakeshore, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada., Martinez Henao J; Department of Plant Science, McGill University, 21,111 rue Lakeshore, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada., van Dam NM; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 52, 04103 Leipzig, Germany.; Institute of Biodiversity, Friedrich Schiller University Jena, 07743 Jena, Germany.; Leibniz Institute for Vegetable and Ornamental Crops (IGZ), Theodor-Echtermeyerweg-1, 14979 Großbeeren, Germany., Rivoal J; Institut de Recherche en Biologie Végétale, Université de Montréal, 4101 rue Sherbrooke E., Montréal, QC H1X 2B2, Canada., Bede JC; Department of Plant Science, McGill University, 21,111 rue Lakeshore, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada. |
| Abstrakt: |
In wounded Arabidopsis thaliana leaves, four 13 S -lipoxygenases (AtLOX2, AtLOX3, AtLOX4, AtLOX6) act in a hierarchical manner to contribute to the jasmonate burst. This leads to defense responses with LOX2 playing an important role in plant resistance against caterpillar herb-ivory. In this study, we sought to characterize the impact of AtLOX2 on wound-induced phytohormonal and transcriptional responses to foliar mechanical damage using wildtype (WT) and lox2 mutant plants. Compared with WT, the lox2 mutant had higher constitutive levels of the phytohormone salicylic acid (SA) and enhanced expression of SA-responsive genes. This suggests that AtLOX2 may be involved in the biosynthesis of jasmonates that are involved in the antagonism of SA biosynthesis. As expected, the jasmonate burst in response to wounding was dampened in lox2 plants. Generally, 1 h after wounding, genes linked to jasmonate biosynthesis, jasmonate signaling attenuation and abscisic acid-responsive genes, which are primarily involved in wound sealing and healing, were differentially regulated between WT and lox2 mutants. Twelve h after wounding, WT plants showed stronger expression of genes associated with plant protection against insect herbivory. This study highlights the dynamic nature of jasmonate-responsive gene expression and the contribution of AtLOX2 to this pathway and plant resistance against insects. |
