| Autor: |
Kaczmarek-Szczepańska B; Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarin 7, 87-100 Torun, Poland., Kleszczyński K; Department of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, Germany., Zasada L; Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarin 7, 87-100 Torun, Poland., Chmielniak D; Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarin 7, 87-100 Torun, Poland., Hollerung MB; Department of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, Germany., Dembińska K; Department of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, 87-100 Torun, Poland., Pałubicka K; Department of Conservation and Restoration of Paper and Leather, Nicolaus Copernicus University in Torun, Sienkiewicza 30/32, 87-100 Torun, Poland., Steinbrink K; Department of Dermatology, University of Münster, Von-Esmarch-Str. 58, 48149 Münster, Germany., Swiontek Brzezinska M; Department of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, 87-100 Torun, Poland., Grabska-Zielińska S; Faculty of Chemical Technology and Engineering, Bydgoszcz University of Science and Technology, Seminaryjna 3, 85-326 Bydgoszcz, Poland. |
| Abstrakt: |
The aim of this work was to develop and characterize a thin films composed of hyaluronic acid/ellagic acid for potential medical application. Its principal novelty, distinct from the prior literature in terms of hyaluronic acid films supplemented with phenolic acids, resides in the predominant incorporation of ellagic acid-a distinguished compound-as the primary constituent of the films. Herein, ellagic acid was dissolved in two different solvents, i.e., acetic acid (AcOH) or sodium hydroxide (NaOH), and the surface properties of the resultant films were assessed using atomic force microscopy and contact angle measurements. Additionally, various physicochemical parameters were evaluated including moisture content, antioxidant activity, and release of ellagic acid in phosphate buffered saline. Furthermore, the evaluation of films' biocompatibility was conducted using human epidermal keratinocytes, dermal fibroblasts, and human amelanotic melanoma cells (A375 and G361), and the antimicrobial activity was elucidated accordingly against Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442. Our results showed that the films exhibited prominent antibacterial properties particularly against Staphylococcus aureus , with the 80HA/20EA/AcOH film indicating the strong biocidal activity against this strain leading to a significant reduction in viable cells. Comparatively, the 50HA/50EA/AcOH film also displayed biocidal activity against Staphylococcus aureus . This experimental approach could be a promising technique for future applications in regenerative dermatology or novel strategies in terms of bioengineering. |
