Effect of Rapid High-Intensity Light-Curing on Increasing Transdentinal Temperature and Cell Viability: An In Vitro Study.

Autor: Miranda SB; School of Dentistry, University of Pernambuco, Recife 50100-130, Pernambuco, Brazil., Lins RBE; School of Dentistry, Federal University of Alagoas, Maceió 57072-900, Alagoas, Brazil., Santi MR; Piracicaba Dental School, University of Campinas, Piracicaba 13414-018, São Paulo, Brazil., Denucci GC; Department of Cariology and Operative Dentistry, Indiana University School of Dentistry, Indianapolis, IN 46202, USA., Silva CCS; Department of Clinical Nurse, Federal University of Paraiba, João Pessoa 58058-600, Paraíba, Brazil., da Silva SFF; School of Biological Sciences, University of Pernambuco, Recife 50100-130, Pernambuco, Brazil., Marques DAV; School of Biological Sciences, University of Pernambuco, Recife 50100-130, Pernambuco, Brazil., Montes MAJR; Department of Dental Materials, University of Pernambuco, Recife 50100-130, Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: Polymers [Polymers (Basel)] 2024 May 22; Vol. 16 (11). Date of Electronic Publication: 2024 May 22.
DOI: 10.3390/polym16111466
Abstrakt: Background: This study investigated effects of rapid high-intensity light-curing (3 s) on increasing transdentinal temperature and cell viability.
Methods: A total of 40 dentin discs (0.5 mm) obtained from human molars were prepared, included in artificial pulp chambers (4.5 × 5 mm), and subjected to four light-curing protocols (n = 5), with a Valo Grand light curing unit: (i) 10 s protocol with a moderate intensity of 1000 mW/cm 2 (Valo-10 s); (ii) 3 s protocol with a high intensity of 3200 mW/cm 2 (Valo-3 s); (iii) adhesive system + Filtek Bulk-Fill Flow bulk-fill composite resin in 10 s (FBF-10 s); (iv) adhesive system + Tetric PowerFlow bulk-fill composite resin in 3 s (TPF-3 s). Transdentinal temperature changes were recorded with a type K thermocouple. Cell viability was assessed using the MTT assay. Data were analyzed using one-way ANOVA and Tukey tests for comparison between experimental groups ( p < 0.05).
Results: The 3 s high-intensity light-curing protocol generated a higher temperature than the 10 s moderate-intensity standard ( p < 0.001). The Valo-10 s and Valo-3 s groups demonstrated greater cell viability than the FBF-10s and TPF-3 s groups and statistical differences were observed between the Valo-3 s and FBF-10 s groups ( p = 0.023) and Valo-3 s and TPF-3 s ( p = 0.025), with a potential cytotoxic effect for the FBF-10 s and TPF-3 s groups.
Conclusions: The 3 s rapid high-intensity light-curing protocol of bulk-fill composite resins caused a temperature increase greater than 10 s and showed cell viability similar to and comparable to the standard protocol.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
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