Evaluation of darbepoetin therapy on platelet count and function in healthy cats and cats with surgically induced chronic kidney disease.

Autor:	De Lombaert MCM; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA., Schmiedt CW; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA., Lourenço BN; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA., Tarigo JL; Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA., Hodges KM; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA., Brainard BM; Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA.
Jazyk:	angličtina
Zdroj:	American journal of veterinary research [Am J Vet Res] 2024 Jun 17; Vol. 85 (8). Date of Electronic Publication: 2024 Jun 17 (Print Publication: 2024).
DOI:	10.2460/ajvr.24.03.0077
Abstrakt:	Objective: To evaluate the effects of darbepoetin on platelet population and reactivity in healthy cats (HCs) and azotemic cats with remnant kidney (RK) model-induced chronic kidney disease. Animals: 12 purpose-bred domestic shorthair cats (n = 6 HCs and n = 6 RK). Methods: In this pilot study, all cats received darbepoetin (1 µg/kg, SC) on days 0, 7, and 14. Blood was sampled at baseline and on days 3, 10, 15, 17, 20, and 21. At each time point, a CBC was performed, platelet aggregometry was assessed by impedance and optical methods, and platelet P-selectin (CD62P) was quantified before and after thrombin stimulation. Additionally, reticulated platelets were quantified using both thiazole orange staining and proprietary analysis by the CBC analyzer. For RK cats, systemic blood pressure (BP) was serially measured. Results: No adverse effects of darbepoetin were seen. There was no statistically significant change in platelet count between or within groups at any time point. Hematocrit increased significantly over time in the RK but not the HC group. RBC reticulocyte numbers in both groups increased over time. Reticulated platelet percentage did not increase in either group. Differences in platelet reactivity within or between groups were not seen in the aggregometry or flow cytometric assessments. In RK cats, indirect BP did not significantly change during the study. Clinical Relevance: This preliminary investigation did not find evidence that darbepoetin administration impacted platelet number, reactivity, nor reticulated platelet count. Anemic RK cats experienced increased hematocrit and RBC reticulocytes as expected with darbepoetin therapy.
Databáze:	MEDLINE
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