Molecular characterization of trypanocide-resistant strains derived from a single field isolate of Trypanosoma evansi.

Autor: Subekti DT; Veterinary Science Program, Faculty of Veterinary Medicine, Airlangga University, Campus C - JL. Mulyorejo, Surabaya, East Java Province 60115, Indonesia; Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Cibinong Science Center, JL. Raya Jakarta - Bogor Km. 46, Bogor, West Jawa Province 16911, Indonesia. Electronic address: subektididik96@yahoo.com., Azmi Z; Center for Standardization of Animal Husbandry and Animal Health Instruments, Agency for Standardization of Agricultural Instruments, Indonesian Ministry of Agriculture, JL. Raya Pajajaran Kav. E No. 59, Bogor, West Jawa Province 16143, Indonesia., Kurniawati DA; Center for Veterinary Instrument Standard Testing (CVIST), Agency for Standardization of Agricultural Instruments, Indonesian Ministry of Agriculture, JL. RE. Martadinata 30, Bogor, West Jawa Province 16114, Indonesia., Suwanti LT; Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University, Campus C - JL. Mulyorejo, Surabaya, East Java Province 60115, Indonesia., Mufasirin M; Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University, Campus C - JL. Mulyorejo, Surabaya, East Java Province 60115, Indonesia., Sunarno S; Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Cibinong Science Center, JL. Raya Jakarta - Bogor Km. 46, Bogor, West Jawa Province 16911, Indonesia.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2024 Aug; Vol. 330, pp. 110236. Date of Electronic Publication: 2024 Jun 15.
DOI: 10.1016/j.vetpar.2024.110236
Abstrakt: Four strains (SB-PR, SB-RS, SB-RD, and SB-RM) of Trypanosoma evansi (T. evansi) were used in this study. SB-PR is known to be trypanocide-sensitive, while the others are trypanocide-resistant to suramin, diminazene diaceturate, and melarsomine hydrochloride, respectively. SB-RS, SB-RD, and SB-RM are derivatives of a single field isolate of SB-PR. Trypanocide resistance will not only increase costs and decrease production efficiency but will also affect effective treatment strategies. Therefore, studies on this topic are important to avoid inefficient production and ineffective treatment. This paper aims to presents a comparative molecular characterization of the trypanocide-resistant strains compared to the parent population. Comparative molecular characterization of these strains based on a protein profile analysis performed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), DNA fingerprinting of random amplified polymorphic DNA (RAPD), and the molecular characterization of expression-site-associated 6 (ESAG6), variant surface glycoprotein (VSG), and T. evansi adenosine transporter-1 (TevAT1) gene sequences. The results show three derived strains (SB-RS, SB-RD, and SB-RM) exhibit different banding patterns than SB-PR. According to the RAPD results, SB-RS and SB-RD are different strains with DNA fingerprint similarities of about 77.8 %, while the DNA fingerprint of SB-RM has a similarity of 44.4 % to SB-RS and SB-RD. No differences in VSG were found among the four strains; however, ESAG6 showed differences in both nucleotide and amino acid sequences, as well as in its secondary and 3D structure. In conclusion, all molecular analyses of the ESAG6 gene showed that SB-PR, SB-RS, SB-RD, and SB-RM are different strains. Furthermore, SB-PR, SB-RS, SB-RD, and SB-RM did not exhibit the TevAT1 gene, so the resistance mechanism was determined to be unrelated to that gene.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Didik T Subekti reports administrative support and equipment, drugs, or supplies were provided by Indonesian Research Center for Veterinary Science. Didik T Subekti reports a relationship with ICenter for Biomedical Research, Research Organization for Health, National Research and Innovation Agency that includes: employment. Didik T Subekti has patent pending to Didik T Subekti. No conflict of interest If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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