A niche-derived nonribosomal peptide triggers planarian sexual development.

Autor:	Issigonis M; Morgridge Institute for Research, University of Wisconsin-Madison, Madison, WI 53715.; Department of Integrative Biology, University of Wisconsin-Madison, Madison, WI 53715., Browder KL; Department of Integrative Biology, University of Wisconsin-Madison, Madison, WI 53715.; HMI, University of Wisconsin-Madison, Madison, WI 53715., Chen R; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390., Collins JJ 3rd; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390., Newmark PA; Morgridge Institute for Research, University of Wisconsin-Madison, Madison, WI 53715.; Department of Integrative Biology, University of Wisconsin-Madison, Madison, WI 53715.; HMI, University of Wisconsin-Madison, Madison, WI 53715.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jun 25; Vol. 121 (26), pp. e2321349121. Date of Electronic Publication: 2024 Jun 18.
DOI:	10.1073/pnas.2321349121
Abstrakt:	Germ cells are regulated by local microenvironments (niches), which secrete instructive cues. Conserved developmental signaling molecules act as niche-derived regulatory factors, yet other types of niche signals remain to be identified. Single-cell RNA-sequencing of sexual planarians revealed niche cells expressing a nonribosomal peptide synthetase ( nrps ). Inhibiting nrps led to loss of female reproductive organs and testis hyperplasia. Mass spectrometry detected the dipeptide β-alanyl-tryptamine (BATT), which is associated with reproductive system development and requires nrps and a monoamine-transmitter-synthetic enzyme Aromatic L-amino acid decarboxylase (AADC) for its production. Exogenous BATT rescued the reproductive defects after nrps or aadc inhibition, restoring fertility. Thus, a nonribosomal, monoamine-derived peptide provided by niche cells acts as a critical signal to trigger planarian reproductive development. These findings reveal an unexpected function for monoamines in niche-germ cell signaling. Furthermore, given the recently reported role for BATT as a male-derived factor required for reproductive maturation of female schistosomes, these results have important implications for the evolution of parasitic flatworms and suggest a potential role for nonribosomal peptides as signaling molecules in other organisms. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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