CBP60b clade proteins are prototypical transcription factors mediating immunity.

Autor:	Li LS; Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tian'jin 300071, China., Yang YY; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Chen YX; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Yu F; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Hao GJ; Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tian'jin 300071, China., Yin GM; Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tian'jin 300071, China., Dou Y; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Zhi JY; Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tian'jin 300071, China., Ma L; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Wang JF; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Feng QN; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China., Zhang Y; Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tian'jin 300071, China., Li S; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2024 Oct 01; Vol. 196 (2), pp. 1489-1501.
DOI:	10.1093/plphys/kiae349
Abstrakt:	Transcriptional reprogramming is critical for plant immunity. Several calmodulin (CaM)-binding protein 60 (CBP60) family transcription factors (TFs) in Arabidopsis (Arabidopsis thaliana), including CBP60g, systemic acquired resistance deficient 1 (SARD1), CBP60a, and CBP60b, are critical for and show distinct roles in immunity. However, there are additional CBP60 members whose function is unclear. We report here that Arabidopsis CBP60c-f, 4 uncharacterized CBP60 members, play redundant roles with CBP60b in the transcriptional regulation of immunity responses, whose pCBP60b-driven expression compensates the loss of CBP60b. By contrast, neither CBP60g nor SARD1 is interchangeable with CBP60b, suggesting clade-specific functionalization. We further show that the function of CBP60b clade TFs relies on DNA-binding domains (DBDs) and CaM-binding domains, suggesting that they are downstream components of calcium signaling. Importantly, we demonstrate that CBP60s encoded in earliest land plant lineage Physcomitrium patens and Selaginella moellendorffii are functionally homologous to Arabidopsis CBP60b, suggesting that the CBP60b clade contains the prototype TFs of the CBP60 family. Furthermore, tomato and cucumber CBP60b-like genes rescue the defects of Arabidopsis cbp60b and activate the expression of tomato and cucumber SALICYLIC ACID INDUCTION DEFICIIENT2 (SID2) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) genes, suggesting that immune response pathways centered on CBP60b are also evolutionarily conserved. Together, these findings suggest that CBP60b clade TFs are functionally conserved in evolution and positively mediate immunity. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu