Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research.
| Autor: | Chiok KR; Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada., Dahlan NA; Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.; Division of Biomedical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada., Banerjee A; Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada.; Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada.; Respiratory Research Centre, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada., Dhar N; Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada. neeraj.dhar@usask.ca.; Respiratory Research Centre, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada. neeraj.dhar@usask.ca.; Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada. neeraj.dhar@usask.ca.; School of Public Health, University of Saskatchewan, Saskatoon, SK, Canada. neeraj.dhar@usask.ca. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2813, pp. 137-144. |
| DOI: | 10.1007/978-1-0716-3890-3_10 |
| Abstrakt: | Air-liquid interface (ALI) airway culture models serve as a powerful tool to emulate the characteristic features of the respiratory tract in vitro. These models are particularly valuable for studying emerging respiratory viral and bacterial infections. Here, we describe an optimized protocol to obtain the ALI airway culture models using normal human bronchial epithelial cells (NHBECs). The protocol outlined below enables the generation of differentiated mucociliary airway epithelial cultures by day 28 following exposure to air. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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