Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance.

Autor:	Li J; Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A., Wyatt NA; Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A.; Sugarbeet and Potato Research Unit, Edward T. Schafer Agricultural Research Center, USDA-ARS, Fargo, ND 58102, U.S.A., Skiba RM; Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, USDA-ARS, Fargo, ND 58102, U.S.A., Kariyawasam GK; Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A., Richards JK; Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, U.S.A., Effertz K; Department of Crop and Soil Science, Washington State University, Pullman, WA 99164, U.S.A., Rehman S; Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat 10100, Morocco.; Field Crop Development Center of the Olds College, Lacombe, Alberta T4L1W8, Canada., Liu Z; Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A., Brueggeman RS; Department of Crop and Soil Science, Washington State University, Pullman, WA 99164, U.S.A., Friesen TL; Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A.; Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, USDA-ARS, Fargo, ND 58102, U.S.A.
Jazyk:	angličtina
Zdroj:	Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2024 Sep; Vol. 37 (9), pp. 676-687. Date of Electronic Publication: 2024 Aug 27.
DOI:	10.1094/MPMI-10-23-0159-R
Abstrakt:	Barley net form net blotch (NFNB) is a destructive foliar disease caused by Pyrenophora teres f. teres. Barley line CIho5791, which harbors the broadly effective chromosome 6H resistance gene Rpt5 , displays dominant resistance to P. teres f. teres . To genetically characterize P. teres f. teres avirulence/virulence on the barley line CIho5791, we generated a P. teres f. teres mapping population using a cross between the Moroccan CIho5791-virulent isolate MorSM40-3 and the avirulent reference isolate 0-1. Full genome sequences were generated for 103 progenies. Saturated chromosome-level genetic maps were generated, and quantitative trait locus (QTL) mapping identified two major QTL associated with P. teres f. teres avirulence/virulence on CIho5791. The most significant QTL mapped to chromosome (Ch) 1, where the virulent allele was contributed by MorSM40-3. A second QTL mapped to Ch8; however, this virulent allele was contributed by the avirulent parent 0-1. The Ch1 and Ch8 loci accounted for 27 and 15% of the disease variation, respectively, and the avirulent allele at the Ch1 locus was epistatic over the virulent allele at the Ch8 locus. As a validation, we used a natural P. teres f. teres population in a genome-wide association study that identified the same Ch1 and Ch8 loci. We then generated a new reference quality genome assembly of parental isolate MorSM40-3 with annotation supported by deep transcriptome sequencing of infection time points. The annotation identified candidate genes predicted to encode small, secreted proteins, one or more of which are likely responsible for overcoming the CIho5791 resistance. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 "No Rights Reserved" license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu