Real-Time Metabolic Magnetic Resonance Spectroscopy of Pancreatic and Colon Cancer Tumor-Xenografts with Parahydrogen Hyperpolarized 1- 13 C Pyruvate-d 3 .

Autor: Fries LM; NMR Signal Enhancement Group Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany., Hune TLK; NMR Signal Enhancement Group Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany., Sternkopf S; NMR Signal Enhancement Group Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany., Mamone S; NMR Signal Enhancement Group Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany.; Present address: Department of Life, Health and Environmental Science, University of L'Aquila, Via Vetoio, Localita' Coppito, 67100, L'Aquila, Italy., Schneider KL; Department of Molecular Oncology, University Medical Center Göttingen, Justus von Liebig Weg 11, 37077, Göttingen, Germany.; Clinical Research Unit 5002, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany., Schulz-Heddergott R; Department of Molecular Oncology, University Medical Center Göttingen, Justus von Liebig Weg 11, 37077, Göttingen, Germany.; Clinical Research Unit 5002, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany., Becker D; Department of NMR-based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077, Göttingen, Germany., Glöggler S; NMR Signal Enhancement Group Max Planck Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077, Göttingen, Germany.; Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, Robert-Koch-Straße 40, 37075, Göttingen, Germany.
Jazyk: angličtina
Zdroj: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2024 Sep 11; Vol. 30 (51), pp. e202400187. Date of Electronic Publication: 2024 Aug 25.
DOI: 10.1002/chem.202400187
Abstrakt: Parahydrogen-induced polarization (PHIP) is an emerging technique to enhance the signal of stable isotope metabolic contrast agents for Magnetic Resonance (MR). The objective of this study is to continue establishing 1- 13 C-pyruvate-d 3 , signal-enhanced via PHIP, as a hyperpolarized contrast agent, obtained in seconds, to monitor metabolism in human cancer. Our focus was on human pancreatic and colon tumor xenografts. 1- 13 C-vinylpyruvate-d 6 was hydrogenated using parahydrogen. Thereafter, the polarization of the protons was transferred to 13 C. Following a workup procedure, the free hyperpolarized 1- 13 C-pyruvate-d 3 was obtained in clean aqueous solution. After injection into animals bearing either pancreatic or colon cancer xenografts, slice-selective MR spectra were acquired and analyzed to determine rate constants of metabolic conversion into lactate and alanine. 1- 13 C-pyruvate-d 3 proved to follow the increased metabolic rate to lactate and alanine in the tumor xenografts.
(© 2024 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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