In Vitro and In Vivo Analyses Reveal Tumor-Derived Exosome miR-558 Promotes Angiogenesis in Tongue Squamous Cell Carcinoma by Targeting Heparinase.

Autor:	Ding B; Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, China.; Nantong University, Nantong, China., Chen Q; Department of Otolaryngology-Head and Neck Surgery, Affiliated Tumor Hospital of Nantong University, Nantong, China., Wu Z; Department of Otolaryngology-Head and Neck Surgery, Changshu Second People's Hospital, Suzhou, China., Li X; Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; Ear Institute, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab, Shanghai, China., Ding Y; Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, China.; Nantong University, Nantong, China., Wu Q; Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, China.; Nantong University, Nantong, China., Han L; Department of Otolaryngology-Head and Neck Surgery, Affiliated Tumor Hospital of Nantong University, Nantong, China., Wu H; Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, China.
Jazyk:	angličtina
Zdroj:	Technology in cancer research & treatment [Technol Cancer Res Treat] 2024 Jan-Dec; Vol. 23, pp. 15330338241261615.
DOI:	10.1177/15330338241261615
Abstrakt:	This study aimed to investigate the role of miR-558 in tumor angiogenesis by targeting heparinase (HPSE) in tongue squamous cell carcinoma (TSCC)-derived exosomes. In the present study, the role of exosome miR-558 in angiogenesis in vitro and in vivo was investigated by cell proliferation, migration, tube formation, subcutaneous tumor formation in mice, and in vivo Matrigel plug assay. The target genes of miR-558 were detected by means of dual luciferase assay. It was found that TSCC cells secrete miR-558 into the extracellular environment, with exosome as the carrier. Human umbilical vein endothelial cells (HUVEC) ingested exosomes, which not only increased the expression level of miR-558, but also enhanced their proliferation, migration, and tube formation functions. In vivo Matrigel plug assay demonstrated that TSCC cell-derived exosome miR-558 promoted neovascularization in vivo. Compared with negative control cells, TSCC cells overexpressing miR-558 formed subcutaneous tumors in nude mice, with larger volume, heavier mass, and more vascularization. Dual luciferase assay confirmed that HPSE was the direct target gene regulated by miR-558. HPSE promoted the proliferation, migration, and tube formation of HUVECs, and the knockout of HPSE could downregulate the pro-angiogenic effect of miR-558. In summary, miR-558 in TSCC exosomes promotes the proliferation, migration, and tube formation of HUVECs by targeting HPSE, and enhancing tumor angiogenesis. Competing Interests: Declaration of Conflicting InterestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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