Genetic association analysis in sugarcane (Saccharum spp.) for sucrose accumulation in humid environments in Colombia.

Autor: Saavedra-Díaz C; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia.; Pontificia Universidad Javeriana, Cali, Colombia., Trujillo-Montenegro JH; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., Jaimes HA; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., Londoño A; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., Villareal FAS; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., López LO; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., Valens CAV; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., López-Gerena J; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., Riascos JJ; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., Quevedo YM; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia., Aguilar FS; Centro de Investigación de la Caña de Azúcar de Colombia (CENICAÑA), Cali, Colombia. fsilvaag@cenicana.org.; Colombian Sugarcane Research Center (Cenicaña), km 26 Vía Cali-Florida, Valle del Cauca, Colombia. fsilvaag@cenicana.org.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 Jun 18; Vol. 24 (1), pp. 570. Date of Electronic Publication: 2024 Jun 18.
DOI: 10.1186/s12870-024-05233-y
Abstrakt: Background: Sucrose accumulation in sugarcane is affected by several environmental and genetic factors, with plant moisture being of critical importance for its role in the synthesis and transport of sugars within the cane stalks, affecting the sucrose concentration. In general, rainfall and high soil humidity during the ripening stage promote plant growth, increasing the fresh weight and decreasing the sucrose yield in the humid region of Colombia. Therefore, this study aimed to identify markers associated with sucrose accumulation or production in the humid environment of Colombia through a genome-wide association study (GWAS).
Results: Sucrose concentration measurements were taken in 220 genotypes from the Cenicaña's diverse panel at 10 (early maturity) and 13 (normal maturity) months after planting. For early maturity data was collected during plant cane and first ratoon, while at normal maturity it was during plant cane, first, and second ratoon. A total of 137,890 SNPs were selected after sequencing the 220 genotypes through GBS, RADSeq, and whole-genome sequencing. After GWAS analysis, a total of 77 markers were significantly associated with sucrose concentration at both ages, but only 39 were close to candidate genes previously reported for sucrose accumulation and/or production. Among the candidate genes, 18 were highlighted because they were involved in sucrose hydrolysis (SUS6, CIN3, CINV1, CINV2), sugar transport (i.e., MST1, MST2, PLT5, SUT4, ERD6 like), phosphorylation processes (TPS genes), glycolysis (PFP-ALPHA, HXK3, PHI1), and transcription factors (ERF12, ERF112). Similarly, 64 genes were associated with glycosyltransferases, glycosidases, and hormones.
Conclusions: These results provide new insights into the molecular mechanisms involved in sucrose accumulation in sugarcane and contribute with important genomic resources for future research in the humid environments of Colombia. Similarly, the markers identified will be validated for their potential application within Cenicaña's breeding program to assist the development of breeding populations.
(© 2024. The Author(s).)
Databáze: MEDLINE
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