Zinc-finger (ZiF) fold secreted effectors form a functionally diverse family across lineages of the blast fungus Magnaporthe oryzae.

Autor: De la Concepcion JC; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Langner T; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Fujisaki K; Division of Genomics and Breeding, Iwate Biotechnology Research Center, Iwate, Japan., Yan X; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Were V; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Lam AHC; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, United Kingdom., Saado I; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, United Kingdom., Brabham HJ; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Win J; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Yoshida K; Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan., Talbot NJ; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Terauchi R; Division of Genomics and Breeding, Iwate Biotechnology Research Center, Iwate, Japan.; Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Kyoto, Japan., Kamoun S; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom., Banfield MJ; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2024 Jun 17; Vol. 20 (6), pp. e1012277. Date of Electronic Publication: 2024 Jun 17 (Print Publication: 2024).
DOI: 10.1371/journal.ppat.1012277
Abstrakt: Filamentous plant pathogens deliver effector proteins into host cells to suppress host defence responses and manipulate metabolic processes to support colonization. Understanding the evolution and molecular function of these effectors provides knowledge about pathogenesis and can suggest novel strategies to reduce damage caused by pathogens. However, effector proteins are highly variable, share weak sequence similarity and, although they can be grouped according to their structure, only a few structurally conserved effector families have been functionally characterized to date. Here, we demonstrate that Zinc-finger fold (ZiF) secreted proteins form a functionally diverse effector family in the blast fungus Magnaporthe oryzae. This family relies on the Zinc-finger motif for protein stability and is ubiquitously present in blast fungus lineages infecting 13 different host species, forming different effector tribes. Homologs of the canonical ZiF effector, AVR-Pii, from rice infecting isolates are present in multiple M. oryzae lineages. Wheat infecting strains of the fungus also possess an AVR-Pii like allele that binds host Exo70 proteins and activates the immune receptor Pii. Furthermore, ZiF tribes may vary in the proteins they bind to, indicating functional diversification and an intricate effector/host interactome. Altogether, we uncovered a new effector family with a common protein fold that has functionally diversified in lineages of M. oryzae. This work expands our understanding of the diversity of M. oryzae effectors, the molecular basis of plant pathogenesis and may ultimately facilitate the development of new sources for pathogen resistance.
Competing Interests: SK receives funding from Industry on NLR biology.
(Copyright: © 2024 Concepcion et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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