Engineered Bacteria as Living Biosensors in Dermal Tattoos.

Autor: Allen ME; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London, W12 0BZ, UK.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub, London, W12 0BZ, UK.; Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, UK.; fabriCELL, Imperial College London and King's College London, London, W12 0BZ, UK., Kamilova E; Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, UK., Monck C; Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, UK., Ceroni F; Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, UK., Hu Y; Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, UK., Yetisen AK; Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, UK., Elani Y; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub, London, W12 0BZ, UK.; Department of Chemical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, UK.; fabriCELL, Imperial College London and King's College London, London, W12 0BZ, UK.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Aug; Vol. 11 (30), pp. e2309509. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1002/advs.202309509
Abstrakt: Dermal tattoo biosensors are promising platforms for real-time monitoring of biomarkers, with skin used as a diagnostic interface. Traditional tattoo sensors have utilized small molecules as biosensing elements. However, the rise of synthetic biology has enabled the potential employment of engineered bacteria as living analytical tools. Exploiting engineered bacterial sensors will allow for potentially more sensitive detection across a broad biomarker range, with advanced processing and sense/response functionalities using genetic circuits. Here, the interfacing of bacterial biosensors as living analytics in tattoos is shown. Engineered bacteria are encapsulated into micron-scale hydrogel beads prepared through scalable microfluidics. These biosensors can sense both biochemical cues (model biomarkers) and biophysical cues (temperature changes, using RNA thermometers), with fluorescent readouts. By tattooing beads into skin models and confirming sensor activity post-tattooing, our study establishes a foundation for integrating bacteria as living biosensing entities in tattoos.
(© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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