Topical Adipose Mesenchymal Stem cell metabolites regulate the expression of MMP-1, MMP-9, EGF, TGF-β in oral mucosa ulcer rat model.

Autor:	Susanto H; Departement of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Indonesia., Ratu Mas Saraswati A; Clinical Dentistry, Magister Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Indonesia., Patera Nugraha A; Departement of Orthodontic, Faculty of Dentistry, Universitas Airlangga, Indonesia., Wicaksono S; Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Indonesia., Nur'aeny N; Departement of Oral Medicine, Faculty of Dentistry, Universitas Padjajaran, Indonesia., Savitri Ernawati D; Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Indonesia.
Jazyk:	angličtina
Zdroj:	The Saudi dental journal [Saudi Dent J] 2024 Jun; Vol. 36 (6), pp. 932-939. Date of Electronic Publication: 2024 Apr 02.
DOI:	10.1016/j.sdentj.2024.03.021
Abstrakt:	Objective: Matrix metalloproteinase (MMP-1 and -9), Epidermal Growth Factor (EGF), and Transforming Growth factor (TGF)-β are expressed in the oral ulcer wound-healing process. The Adipose mesenchymal stem cell metabolites (AdMSCMs) may accelerate wound-healing. This study aimed to investigate the expression of MMP-1, MMP-9, EGF, and TGF-β in the oral mucosa ulcer rat model treated with topical AdMSCMs. Materials and Methods: Oral ulcer models were created in twenty healthy male Wistar rats ( Rattus novergicus ) divided into AdMSCMs and control groups. The oral ulcer model was treated topically using AdMSCMs oral gel three times daily for 3 and 7 days. The expression of MMP-1, MMP-9, EGF, TGF-β was evaluated through histological examination using the immunohistochemistry method. Independent t -test was used to compare the mean of expression of MMP-1, MMP-9, EGF, TGF-β between control and treatment groups (AdMSCMs), and paired t -test was used to analyze the mean between day 3 and day 7 of each group. Results: A lower expression of MMP-1, MMP-9 in AdMSCMs group and higher expression EGF and TGF-β in AdMSCMs group compared to the control group in day 3 and day 7. Independent t -test results showed a significant difference in the expression of MMP-1, MMP-9, EGF between the control and AdMSCMs group in day 3 and day 7. Only TGF-β expression mean difference between day 3 and day 7 showed a significant difference compared to the control groups (p < 0.05). Conclusions: AdMSCMs oral gel may accelerate oral ulcer healing models by reducing the expression MMP-1, MMP-9, and increasing EGF and TGF-β expressions during the wound-healing process. (© 2024 THE AUTHORS.)
Databáze:	MEDLINE
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