Electrochemical immunomagnetic assay for interleukin-6 detection in human plasma.
| Autor: | Buckey G; Department of Chemistry, Vanderbilt University 7330 Stevenson Center, VU Station B351822 Nashville TN 37235-1822 USA d.cliffel@vanderbilt.edu., Owens OE; Department of Chemistry, Vanderbilt University 7330 Stevenson Center, VU Station B351822 Nashville TN 37235-1822 USA d.cliffel@vanderbilt.edu., Richards HA; Department of Chemistry, Vanderbilt University 7330 Stevenson Center, VU Station B351822 Nashville TN 37235-1822 USA d.cliffel@vanderbilt.edu., Cliffel DE; Department of Chemistry, Vanderbilt University 7330 Stevenson Center, VU Station B351822 Nashville TN 37235-1822 USA d.cliffel@vanderbilt.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Sensors & diagnostics [Sens Diagn] 2024 May 08; Vol. 3 (6), pp. 1039-1043. Date of Electronic Publication: 2024 May 08 (Print Publication: 2024). |
| DOI: | 10.1039/d4sd00058g |
| Abstrakt: | An electrochemical immunoassay for interleukin-6 (IL-6) was developed based on IL-6 capture using magnetic beads and electrochemical signal production using horseradish peroxidase/tetramethylbenzidine. We achieved IL-6 detection from the 50-1000 pg mL -1 range, which is a physiologically relevant IL-6 range for a variety of biological systems. The sandwich assay performed well in phosphate buffered solution as well as in cellular media and human plasma spiked with IL-6, and decreased time to IL-6 concentration readout to approximately one hour. There is also future potential to apply this assay to real-time point-of-care human disease diagnostics. Competing Interests: The authors declare no conflicts of interest. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
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