Conjoint analysis of physio-biochemical, transcriptomic, and metabolomic reveals the response characteristics of solanum nigrum L. to cadmium stress.

Autor: Wang J; Guizhou Academy of Sciences, Guiyang, Guizhou, 550001, China.; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.; The Land Greening Remediation Engineering Research Center of Guizhou Province, Guiyang, 550001, China., Chen X; Biofuels Institute, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China., Chu S; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China., Hayat K; Key Laboratory of Pollution Exposure and Health Intervention, Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou, 310015, China., Chi Y; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China., Liao X; Guizhou Academy of Sciences, Guiyang, Guizhou, 550001, China.; The Land Greening Remediation Engineering Research Center of Guizhou Province, Guiyang, 550001, China.; Guizhou University, Guiyang, 550025, China., Zhang H; Guizhou Academy of Sciences, Guiyang, Guizhou, 550001, China.; The Land Greening Remediation Engineering Research Center of Guizhou Province, Guiyang, 550001, China., Xie Y; Guizhou Academy of Sciences, Guiyang, Guizhou, 550001, China. xieyuangui12@126.com.; The Land Greening Remediation Engineering Research Center of Guizhou Province, Guiyang, 550001, China. xieyuangui12@126.com., Zhou P; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China., Zhang D; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. zhdsjtu@sjtu.edu.cn.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 Jun 17; Vol. 24 (1), pp. 567. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1186/s12870-024-05278-z
Abstrakt: Cadmium (Cd) is a nonessential element in plants and has adverse effects on the growth and development of plants. However, the molecular mechanisms of Cd phytotoxicity, tolerance and accumulation in hyperaccumulators Solanum nigrum L. has not been well understood. Here, physiology, transcriptome, and metabolome analyses were conducted to investigate the influence on the S. nigrum under 0, 25, 50, 75 and 100 µM Cd concentrations for 7 days. Pot experiments demonstrated that compared with the control, Cd treatment significantly inhibited the biomass, promoted the Cd accumulation and translocation, and disturbed the balance of mineral nutrient metabolism in S. nigrum, particularly at 100 µM Cd level. Moreover, the photosynthetic pigments contents were severely decreased, while the content of total protein, proline, malondialdehyde (MDA), H 2 O 2 , and antioxidant enzyme activities generally increased first and then slightly declined with increasing Cd concentrations, in both leaves and roots. Furthermore, combined with the previous transcriptomic data, numerous crucial coding-genes related to mineral nutrients and Cd ion transport, and the antioxidant enzymes biosynthesis were identified, and their expression pattern was regulated under different Cd stress. Simultaneously, metabolomic analyses revealed that Cd treatment significantly changed the expression level of many metabolites related to amino acid, lipid, carbohydrate, and nucleotide metabolism. Metabolic pathway analysis also showed that S. nigrum roots activated some differentially expressed metabolites (DEMs) involved in energy metabolism, which may enhance the energy supply for detoxification. Importantly, central common metabolism pathways of DEGs and DEMs, including the "TCA cycle", "glutathione metabolic pathway" and "glyoxylate and dicarboxylate metabolism" were screened using conjoint transcriptomics and metabolomics analysis. Our results provide some novel evidences on the physiological and molecular mechanisms of Cd tolerance in hyperaccumulator S. nigrum plants.
(© 2024. The Author(s).)
Databáze: MEDLINE
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